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上海研盟生物科技有限公司Anti-Minibrain抗体*，主要应用于WB、IHC、IF、ELISA、流式细胞术等实验中。说明书随货发送，您也可以直接我司在线客服索取。客服
英文名称：Anti-Minibrain antibody
中文名称：Minibrain 抗体
Minibrain 抗体保存条件：Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C.
★研盟生物★Minibrain 抗体,Anti-Minibrain抗体
石蜡切片的免疫组化SP法参考步骤：
1.60℃烤片2小时
2.脱蜡。二甲苯脱蜡15分钟，三次
3.水化。依次经过无水乙醇、95%、90%、80%、70%，各5分钟，蒸馏水（或自来水）5分钟
4.PBS洗5分钟，三次
5.抗原修复。枸橼酸缓冲液（约92-95℃）煮沸（沸水浴）修复15分钟，自然凉至室温。PBS洗5分钟，三次
6.3%双氧水消化内源性的过氧化物酶，20分钟，PBS洗5分钟，三次
7.正常山羊血清封闭，37℃20分钟
8.一抗孵育，4℃过ye.
9.PBS洗5分钟，三次
10.二抗孵育，37℃20分钟（二抗为*标记的羊抗兔IgG），PBS洗5分钟，三次
11.三抗孵育，37℃20分钟（三抗为辣根酶标记的链霉亲和素），PBS洗5分钟，三次
12.DAB显色，镜下观察结果，适时终止
13.苏木素复染5分钟，自来水冲洗片刻，1%的盐酸酒精（75%）溶液（请自行配置，配制比例为：100ml 75%酒精+1ml浓盐酸）分化30秒，自来水冲洗5分钟蓝化
14.脱水。依次经过70%、80%、90%、95%、无水乙醇，各5分钟，二甲苯15分钟，三次
15.封片，中性树胶封片
Minibrain 抗体,Anti-Minibrain抗体溶解方法:
方法1：我们的抗体（冻干粉）已经包含了0.01M PBS、1% BSA和0.1% 防腐剂（叠氮钠或*），您只需要加入灭菌的双蒸水就行了。抗体溶解后，置于-20℃保存，分装后使用，避免反复冻融，可保证至少1年有效；
方法2：也可以只加入一半体积的双蒸水，再用一半体积的甘油补足，置于-20℃保存，这样抗体不会冻，有利于抗体的稳定。
后续试验时，再使用对应的缓冲液稀释成工作液，即用即配。
我们的抗体（冻干粉），在常温放置下，至少一个月有效；若在-20℃下保存（*），至少三年有效。
Antibodies are prepared with 0.01M PBS, pH 7.4 with 1% BSA and 0.1% Sodium Azide, prior to lyophilization. Currently, we offer both lyophilized and reconstituted antibodies, both resulting in final concentration of 1mg/mL. We recommend two ways to reconstitute antibodies in lyophilized form:
1) Reconstituting by directly adding 100uL of sterile, distilled water.
2) Alternatively, if glycerol does not affect the experiment, add 50uL sterile distilled water, followed by adding 50uL of glycerol.
简单介绍：
产品别名：MNB_DROME; Serine/threonine-protein kinase minibrain; mnb; Dmel_CG42273; Dmel_CG7826; Dmel_CG7835; CG42273; CG7826; CG7835; Dm1; Dmel\CG42273; Dyrk1; DYRK1; ME-IV; min; Mnb; MNB.
抗体来源：Rabbit or Mouse
保质期:1年
克隆类型：Polyclonal or monoclonal
性 状：Lyophilized or Liquid
浓 度：1mg/1ml
背景介绍：This gene encodes a member of the Dual-specificity tyrosine phosphorylation-regulated kinase (DYRK) family. This member contains a nuclear targeting signal sequence, a protein kinase domain, a leucine zipper motif, and a highly conservative 13-consecutive-histidine repeat. It catalyzes its autophosphorylation on serine/threonine and tyrosine residues. It may play a significant role in a signaling pathway regulating cell proliferation and may be involved in brain development. This gene is a homolog of Drosophila mnb (minibrain) gene and rat Dyrk gene. It is localized in the Down syndrome critical region of chromosome 21, and is considered to be a strong candidate gene for learning defects associated with Down syndrome. Alternative splicing of this gene generates several transcript variants differing from each other either in the 5' UTR or in the 3' coding region. These variants encode at least five different isoforms.
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