溶酶体荧光探针：
Invitrogen公司原装绿色探针L7526-LysoTracker® Green DND-26 - Special Packaging：LysoTracker® Green DND-26 是对活细胞中的酸性区室进行染色的一种绿色荧光染料，具有 504/511 nm 的zui大激发/发射波长。
Bovine pulmonary artery endothelial cells. MitoFluor™ Far Red 680, LysoTracker® Green DND-26 and Hoechst 33342. Live bovine pulmonary artery endothelial cells were incubated simultaneously with MitoFluor™ Far Red 680 (Cat. no. M22423), LysoTracker® Green DND-26 (Cat. no. L7526) and Hoechst 33342 (Cat. no. H1399, H3570, H21492). MitoFluor™ Far Red 680 accumulated in mitochondria, and its fluorescence (emission maximum ?700 nm) was pseudocolored red. Green-fluorescent LysoTracker® Green dye accumulated in the lysosomes, and blue-fluorescent Hoechst 33342 dye stained the nuclei. The multiple-exposure image was acquired with filters appropriate for fluorescein, DAPI and MitoFluor™ Far Red dye.
常用规格
| Sub-Cellular Localization: | Lysosomes |
| Color: | Green |
| Platform: | Fluorescence Microscope |
| Product Size: | 20 x 50 µL |
| Detection Method: | Fluorescent |
| Excitation⁄Emission (nm): | 504⁄511 |
| Shipping Condition: | Room Temperature |
| Regulatory Statement: | For Research Use Only. Not for use in diagnostic procedures. |
化学结构
LysoTracker® Green DND-26
INGREDIENT A: LysoTracker® Green DND-26
分子式
C18H26BClF2N4O
分子量
398.6894
CAS名称/编号
N/A
荧光光谱
LysoTracker Green
Fluorescence Ex/Em spectra of LysoTracker® Green DND-26 in pH 5.2 buffer.
| L7526 | LysoTracker® Green DND-26 - Special Packaging | 20*50ul | 3610 |
| 50ul | 350 |
红色探针：L7528-LysoTracker® Red DND-99 - Special Packaging:LysoTracker®Red DND-99是标记和示踪活细胞中酸性细胞器的红色荧光染料
LysoTracker®的所有特征探针包括:
• 酸性细胞器的高选择性
• 可在乙醛固定后稳定保持
• 纳摩尔浓度水平标记活细胞的有效性较高
• 活细胞成像
• 激发光/发射光577/590 nm
简单、高特异性、一步法染色和酸性细胞器示踪
LysoTracker&re;探针由一种连接一个弱碱基的荧光基团组成，其中仅弱碱可部分提供质子，以维持PH在中性值。这使LysoTracker®探针能自由无阻地渗透细胞膜，使其能够标记活细胞。LysoTracker®探针对酸性细胞器有高选择性，与二硝基苯(DNP)不同，LysoTracker®探针不需二抗来检测。
LysoTracker®探针可用于多种颜色荧光检测
| L7528 | LysoTracker® Red DND-99 - Special Packaging | 20*50ul | 3565 |
| 50ul | 350 |
