The streptococcal group carbohydrates of streptococcal group B is a complex antigen with a rhamnose oligosaccharide and differing side chains, consisting primarily of glucosamine, either acetylated or non-acetylated. In the PathoDx procedure, specific antibody on latex particles reacts with, and agglutinates, streptococcal group antigen extracted from the bacterial cell wall. The test is intended for use with streptococcal colonies that are betahemolytic on sheep blood agar or for use with pure streptococcal isolates in broth cultures. The reagents are designed to give a positive (2+ or greater) agglutination with one to two 18 to 24 hour colonies for most beta-hemolytic streptococcal isolates.
- Ready-to-use – Prepared reagents
- Easy-to-store – Store at 2-8°C
- Easy to interpret – The PathoDx Strep Grouping kit is designed to give a 2+ or greater agglutination reaction with the extract of one or two colonies of an 18 to 24 hour culture of streptococci of Lancefield group B (large colony variety) in 60 seconds for most streptococcal isolates. In a negative test, a uniform milky appearance with no agglutination after 60 seconds.
Strep B Grouping Latex (Pink Cap) contains 3.0mL of a suspension of synthetic blue latex particles coated with rabbit antibody IgG-sensitized with 0.098% sodium azide and 0.05% ProClin 300® as preservatives.
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Remel™ and Oxoid™ products are now part of the Thermo Scientific brand.
- El Kholy, A., et al. 1974. Appl. Microbiol. 28:836-839.
- Hryniewicz, W., et al. 1976. J. Clin. Microbiol. 4:28-31.
