Use PRO Kit for identification of Candida albicans and Clostridium difficile. C. albicans is the yeast pathogen most frequently isolated from clinical specimens1. In 1990, Perry et al. evaluated a rapid test to aid in the identification of C. albicans based on colorimetric detection of the enzyme, L-proline aminopeptidase2. C. difficile is recognized as the major cause of antibiotic-associated pseudomembranous colitis and a frequent cause of hospital-acquired diarrhoea. Fedorko and Williams reported detection of L-Proline aminopeptidase is useful for identification of C. difficile because it is one of the few biochemical tests for which this organism is positive3.
- Easy to store - No special storage required
- Easy to read - After addition of one drop of Cinnamaldehyde a color change from red to pink color development indicates a positive test
The PRO disk contains the substrate L-proline-β-naphthylamide. This substrate is hydrolyzed by an aminopeptidase releasing β-naphthylamine. After addition of Cinnamaldehyde Reagent, β-naphthylamine reacts with ρ- dimethyl-aminocinnamaldehyde to form a red-colored complex.
- Perry, J.L. and G.R. Miller. 1987. J. Clin. Microbiol. 25:2424-2425.
- Perry, J.L., G.R. Miller, and D.L. Carr. 1990. J. Clin. Microbiol. 28:614-615.
- Fedorko, D.P. and E.C. Williams. 1997. J. Clin. Microbiol. 35:1258-1259.
