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1. What should I know about the stability of your protein products?
Unless otherwise mentioned on the product information sheet, all of our products are formulated in such a manner that the lyophilized proteins are very stable at room temperature. However, we recommend storing lyophilized products at -20o C.
For reconstituted solutions of most products, we recommend short-term storage at 4o C. For longer term storage the protein solution should first be aliquoted (to avoid more than one freeze/thaw cycle) and stored frozen at -20o C. Please keep in mind that every freeze/thaw cycle may cause some denaturation of the protein.
2. Why can't I see the protein pellet in the vial?
Unlike many protein products available on the market, PeproTech products are not formulated with carrier protein or other additives (e.g., BSA, HSA, sucrose, etc.) and are often lyophilized with a minimum amount of salt. As a result, the small amounts of protein can be deposited on the vial during lyophilization as a thin and, sometimes, invisible film. Before opening, we recommend centrifuging each vial in a microcentrifuge for 20-30 seconds to drive any protein that may be lodged in the cap or on the side to the bottom of the vial. Our quality control procedures assure that each vial contains the correct amount of product.
3. What is the relationship between the specific activity expressed as an ED50 and as units/mg?
The ED50 is defined as the cytokine concentration at which the activity is 50% of the maximum response. This method of expressing potency should only be used for cytokines whose dose-response curves are sigmoidal in shape. The formula for converting the activity as an ED50 in ng/ml to specific activity in units/mg is
4. Which cytokines show cross-species activity?
With a few exceptions, most human cytokines are active on mouse cells. Many mouse cytokines are active on human cells, but may show lower specific activity than the corresponding human cytokine. A few human cytokines, such as IL-7, even exhibit higher specific activities on mouse cells than do the corresponding mouse cytokines. The interferons, GM-CSF, IL-3, and IL-4 are known to be species-specific with very little, if any, activity on non-homologous cells. In contrast, the FGF's and neurotrophins are very highly conserved and show excellent activity on cells of other animal species.了解更多详细信息
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