小鼠L苯*解氨酶elisa kit代理(PAL)ELISA Kit 试剂盒
原理 : This as say employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for NGF has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any NGF present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for NGF is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of NGF bound in the initial step. The color development is stopped and the intensity of the color is measured.
特异性: This assay has high sensitivity and excellent specificity for detection of Human NGF. No significant cross-reactivity or interference between Human NGF and analogues was observed.
精密度: Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV%<10%
Three samples of known concentration were tested in twenty assays to assess.
小鼠L苯*解氨酶elisa kit代理(PAL)ELISA Kit 试剂盒
样本搜集及储存: Serum: Use a serum separator tube (SST) and allow samples to clot for two hours at room temperature or overnight at 4°C before centrifugation for 15 minutes at 1000 ×g. Remove serum and assay immediay or aliquot and store samples at -20°C or -80°C. Avoid repeated freeze-thaw cycles.
Plasma: Collect plasma using EDTA, or heparin as an anticoagulant. Centrifuge for 15 minutes at 1000 ×g at 2-8°C within 30 minutes of collection. Assay immediay or aliquot and store samples at -20°C or -80°C. Avoid repeated freeze-thaw cycles.
小鼠L苯*解氨酶elisa kit代理(PAL)ELISA Kit 试剂盒
产品都经过质量检测,*保证质量,如果有个别万一出现质量问题,无条件退货、换货.
elisa检测试剂盒kit,实时价格和产品详细说明,敬请!
主要成分:酶标板,试剂,标准品等
性状:盒装液体
适应:科研实验
有效期:6个月
保存温度: 2-8℃
对外承揽试验:免疫组化,荧光,Tunel,Elisa,WB,
原位杂交,比色法,HE染色,特染,图像分析,!
(北京雅安达生物技术有限公司)