Biotin RNA Labeling Mix40 μl (20 reactions)(Roche) 编号:11685597910
免费代测ELISA试剂盒一周内出结果
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主营产品: ELISA试剂盒,生物试剂,免疫组学,生物抗体,蛋白组学,分子生物等。
货号:11685597910
规格:40 µl (20 reactions)
产地:Roche
价格:1748
Contents
Labeling Efficiency
A standard labeling reaction with 1 µg linear template DNA and an RNA polymerase produces approx. 10 µg of full-length, biotin-labeled RNA. In a spot test, a combination of anti-biotin-AP and the chemiluminescence substrate CSPD can detect as little as 0.3 pg of the biotin-labeled RNA. Solution, 10x conc., pH 7.5
Application
This labeling mix can be used (with SP6, T3, or T7 RNA polymerase) to add a nonradioactive label to RNA during in vitro transcription. Microarray hybridization. The labeled RNA can easily and safely be detected (e.g ., with a fluorescein-conjugated or enzyme-conjugated streptavidin). RNase protection experiments, In situ hybridization.了解更多详细信息
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