Sfi I from Streptomyces fimbriatus1,250 U （10 U/μl）（Roche）编号：11288024001

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Source: From Streptomyces fimbriatus
Special incubation temperature: 50°C
Product Description
Heat inactivation: Sfi I cannot be heat inactivated by 15 min incubation at 65°C.
Compatible ends: For an Sfi I end to be compatible and ligatable with any other end, the two corresponding recognition sites must have contained the same three internal Ns.
Unit definition: One unit is the enzyme activity that compley cleaves 1 µg Ad2 DNA in 1 h at 50°C in a total volume of 25 µl SuRE/Cut buffer M.
Application

Isoschizomers: The enzyme has no known isoschizomers. Because of its unusual recognition sequence, Sfi I is useful for analyzing and cloning large DNA fragments. Methylation sensitivity: Sfi I is inhibited by the presence of 5-methylcytosine at the most central C residue （*） on the recognition sequence. 5-methylcytosine at the other C-positions （°） will not inhibit the enzyme.了解更多详细信息

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