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技术文章

Anti- NOX2/gp91phox

阅读:252发布时间:2015-6-3

Anti- NOX2/gp91phox

 

Catalog Number : xy-10910R         

Quantity size :   0.2ml (dilute with pH 7.4    0.01 M PBS or antibody diluent )

 

Background:    

    NOX2/gp91phox is a critical component of the membrane-bound oxidase of phagocytes that generates superoxide. It is the terminal component of a respiratory chain that transfers single electrons from cytoplasmic NADPH across the plasma membrane to molecular oxygen on the exterior. It also functions as a voltage-gated proton channel that mediates the H(+) currents of resting phagocytes. It participates in the regulation of cellular pH and is blocked by zinc. Defects in CYBB are a cause of X-linked chronic granulomatous disease (X-CGD). X-CGD is characterized by the failure of activated phagocytes to generate superoxide. Patients suffer from life-threatening bacterial/fungal infections.  

 

pecificity :   

         anti- NOX2/gp91phox is a rabbit monoclonal antibody, unconjugated                

         specific for NOX2/gp91phox of human,Rat and mouse                   

         use for western blotting,elisa,immunoprecipitation and immunohistochemistry

         Protein A affinity chromatography purification, purity :>95%

         Isotype: IgG

         mol wt: 63kDa 

 

Anti- NOX2/gp91phox

Application :  

         Western blotting 1:100-500                

         Immunohistochemistry 1:100-500                 

         ELISA 1:500-1000                  

         Optimal working dilutions must be determined by the end user.

 

Storage: 

    Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20℃. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody, the antibody is stable for at least six weeks at 2-4 ℃

 

Important Note:

    This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.


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