脱氧核糖核酸酶Ⅱ（猪脾）资料说明

英文名称：DNase II ；Deoxyribonuclease Ⅱ(porcine spleen)；Deoxyribonucleate 3′-oligonucleotido-hydrolase
其他名称：去氧细胞核酸酶；氧核糖核酸-5ˊ-寡核苷酸水解酶；无核糖核酸酶；脱氧核糖核酸去聚合酶；脱羟核糖核酸酶(猪胰)；除氧细胞核酸酶(猪胰) ；DNA酶
CAS号：9025-64-3
级别：BR 
类型：Type IV
活力：2000～6000 units/mg
活力定义：One Kunitz unit will produce a ΔA260 of 0.001 per min per mL at pH 4.6 at 25℃； [Mg2+] = 0.83 mM
性状(以下信息仅供参考)：白色或类白色冻干粉。essentially salt-free。为双链特异性内切核酸酶，能降解双链DNA成为具有5ˊ-磷酸及3ˊ-OH末端的寡脱氧核糖核苷酸。当有一价锰离子存在时，亦能降解单链DNA。溶于水，微溶于30％乙醇和丙酮。酶反应：脱氧核糖核酸→二核苷酸5′磷酸+寡聚核苷酸5′磷酸zui适pH 7.8。吸光系数(E1%280)11.1。激活剂为二价金属离子，zui大激活可以从镁(Mg2+)+钙(Ca2+)得到。抑制剂有EDTA和十二烷基硫酸钠。可加5mmol/L钙(Ca2+)
用途：本品仅供科研，不得用于其它用途。(以下用途仅供参考)用于分子生物学研究。
保存：-20℃
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