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人β亚基(β HCG)抗体英文名称 hCG beta
中文名称 人β亚基(β HCG)抗体
别 名 HCG beta; Beta-HCG; Anterior pituitary glycoprotein hormones common subunit alpha; beta-HCG; CG ALPHA; CG beta a; CG beta; CGA; CGB; CGB1; CGB2; CGB3; CGB5; CGB7; CGB8; Choriogonadotropin alpha chain; Choriogonadotropin subunit beta; Chorionic gonadotrophin alpha subunit; Chorionic gonadotrophin chain beta; Chorionic gonadotropin alpha polypeptide; Chorionic gonadotropin beta 5 subunit; Chorionic gonadotropin beta 7 subunit; Chorionic gonadotropin beta 8 subunit; Chorionic gonadotropin beta chain; Chorionic gonadotropin beta polypeptide 1; Chorionic gonadotropin beta polypeptide 2; Chorionic gonadotropin beta polypeptide 5; Chorionic gonadotropin beta polypeptide 7; Chorionic gonadotropin beta polypeptide; Chorionic gonadotropin beta subunit; CGHB_HUMAN
研究领域 肿瘤 免疫学
抗体来源 Rabbit
克隆类型 Polyclonal
交叉反应 Human,
人β亚基(β HCG)抗体产品应用 WB=1:100-500 ELISA=1:500-1000 IP=1:20-100 IHC-P=1:100-500 IHC-F=1:100-500 IF=1:100-500 (石蜡切片需做抗原修复)
not yet tested in other applications.
optimal dilutions/concentrations should be determined by the end user.
分 子 量 16kDa
细胞定位 分泌型蛋白
性 状 Lyophilized or Liquid
浓 度 1mg/1ml
免 疫 原 KLH conjugated synthetic peptide derived from human Beta-HCG
亚 型 IgG
纯化方法 affinity purified by Protein A
储 存 液 0.01M PBS, pH 7.4 with 10 mg/ml BSA and 0.1% Sodium azide
保存条件 Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C.
人β亚基(β HCG)抗体产品介绍 background:
This gene is a member of the glycoprotein hormone beta chain family and encodes the beta 3 subunit of chorionic gonadotropin (CG). Glycoprotein hormones are heterodimers consisting of a common alpha subunit and an unique beta subunit which confers biological specificity. CG is produced by the trophoblastic cells of the placenta and stimulates the ovaries to synthesize the steroids that are essential for the maintenance of pregnancy. The beta subunit of CG is encoded by 6 genes which are arranged in tandem and inverted pairs on chromosome 19q13.3 and contiguous with the luteinizing hormone beta subunit gene. [provided by RefSeq].
Function:
Stimulates the ovaries to synthesize the steroids that are essential for the maintenance of pregnancy.
Subunit:
Heterodimer of a common alpha chain and a unique beta chain which confers biological specificity to thyrotropin, lutropin, follitropin and gonadotropin.
Subcellular Location:
Secreted.
Tissue Specificity:
Placenta.
Similarity:
Belongs to the glycoprotein hormones subunit beta family.
Database links:
Entrez Gene: 1082 Human
Entrez Gene: 93659 Human
Entrez Gene: 94027 Human
Entrez Gene: 94115 Human
Omim: 118860 Human
SwissProt: P01233 Human
SwissProt: Q6NT52 Human
Unigene: 172944 Human
Unigene: 446683 Human
Unigene: 590955 Human
Unigene: 659014 Human
Unigene: 681647 Human
Unigene: 743279 Human
Unigene: 745468 Human
Important Note:
This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.
HCG是由胎盘合体滋养层细胞产生的一种糖蛋白激素,HCG-β主要用于胎盘滋养细胞和生殖细胞肿瘤如葡萄胎、绒癌的诊断和辅助诊断。
产品图片
Antigen: bs-0953P, 0.2ug/100ul
Primary: Antiserum, 1:500, 1:1000, 1:2000, 1:4000, 1:8000, 1:16000, 1:32000;
Secondary: HRP conjugated Goat-Anti- Rabbit IgG(bs-0295G-HRP) at 1: 5000;
TMB(C-0024) staining;
Read the data in MicroplateReader by 450nm.
Tissue/cell: human lung tissue; 4% Paraformaldehyde-fixed and paraffin-embedded;
Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min;
Incubation: Anti-Beta-HCG/HCG beta Polyclonal Antibody, Unconjugated(bs-0953R) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining
Tissue/cell: human placenta tissue; 4% Paraformaldehyde-fixed and paraffin-embedded;
Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min;
Incubation: Anti-Beta-HCG/HCG beta Polyclonal Antibody, Unconjugated(bs-0953R) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining
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