细胞名称

Beta-TC-6小鼠胰岛素瘤胰岛β细胞

描述

这株细胞来源于转基因小鼠中生长的一个胰肿瘤(胰岛素瘤)。 这种小鼠携带了大鼠胰岛素II基因启动子调控的SV40早期基因的假基因结构。 细胞包含丰富的胰岛素和小量的胰高血糖素及*。 响应葡萄糖而分泌胰岛素[PubMed:7533732]。

动物种别

小鼠

性别

***

组织来源

胰；胰岛素瘤；β细胞

形态

上皮细胞样

培养基和添加剂

DMEM培养基(GIBCO,货号12800017，添加NaHCO3 1.5g/L)，85%；优质热灭活胎牛血清，15%。 气相：空气，95%；二氧化碳，5%。 温度：37摄氏度。

供应限制

A。仅供研究之用。

REFERENCE

Laurance ME , et al. Glucose responsive insulin secreting beta-cell lines and method for producing same. US Patent 5,773,255 dated Jun 30 1998 Poitout V , et al. Morphological and functional characterization of beta TC-6 cells--an insulin-secreting cell line derived from transgenic mice. Diabetes 44: 306-313, 1995. PubMed: 7533732 Poitout V , et al. Insulin-secreting cell lines: classification, characteristics and potential applications. Diabetes Metab. 22: 7-14, 1996. PubMed: 8697299 Hay, R. J., Caputo, J. L., and Macy, M. L., Eds. (1992), ATCC Quality Control Methods for Cell Lines. 2nd edition, Published by ATCC. Caputo, J. L., Biosafety procedures in cell culture. J. Tissue Culture Methods 11:223-227, 1988. Fleming, D.O., Richardson, J. H., Tulis, J.J. and Vesley, D., (1995) Laboratory Safety: Principles and Practice. Second edition, ASM press, Washington, DC. Centers for Disease Control (1993), Biosafety in Microbiological and Biomedical Laboratories Human Health Service Publication No. (CDC) 93-8395. U.S. Dept. of Health and Human Services; 3rd Edition U.S. Government Printing Office Washington D.C.