细胞名称

AML12小鼠肝细胞AML12（干细胞库保藏）

描述

小鼠肝实质细胞，每支细胞量1×10⁶

动物种别

小鼠，CD1品系

性别

雄

组织来源

3月龄的小鼠肝脏

形态

上皮细胞

培养基和添加剂

小鼠肝实质细胞AML12*培养液配方（100 mL）： DMEM/F-12 (Invitrogen, 11330) 89 ml ITS Liquid Media Supplement (Sigma, I3146) 1 ml Dexamethasone 40 ng/ml FBS (Biochrom, S4115) 10 ml 气相：空气，95%；二氧化碳，5%。温度：37摄氏度。

供应限制

REFERENCE

Wu JC, et al. Establishment and characterization of differentiated, nontransformed hepatocyte cell lines derived from mice transgenic for transforming growth factor alpha. Proc. Natl. Acad. Sci. USA 91: 674-678, 1994. PubMed: 7904757 Dumenco L, et al. Introduction of a murine p53 mutation corresponding to human codon 249 into a murine hepatocyte cell line results in growth advantage, but not in transformation. Hepatology 22: 1279-1288, 1995. PubMed: 7557882