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①、取回的样本如宫颈刮片棉签,用2~3ml生理盐水冲洗,可静止30分钟后去掉上层清液,留下层沉淀,加入等体积生理盐水,取样,涂片,也可以离心去上清进行操作;
②、离心之前先将离心机中的离心管套全部取出,先平衡托盘天平(保证指针位于正中),选取相近重量的离心管套,放入样本管,用生理盐水配平;
③、将平衡好的离心管套对称放置,(内圈6孔,外圈6孔,一般先放内孔),一般500转/分,离心3~5分钟即可,如果需要将所有细胞沉淀,可以3000~3500转/分,离心5分钟;本实验500转/分,离心3分钟;
④、离心之后吸去上清,可以留少许上清,混匀后直接取样进行涂片,或者吸去全部上清,再加入等量的生理盐水,混匀后取样涂片;
⑤、取样3~5ul,用枪头进行涂片,涂成1cm2面积的圆形,贴上标签(编号并注明信息,同时可区别正反面,防止将样本擦掉,);
⑥、待样本基本干透之后,可白片进行镜检,观察样本是否干净,涂片是否均匀,(如粘液依旧较多,可以重复生理盐水冲洗和离心、去上清步骤)
⑦、用95%乙醇固定涂片2~3分钟,蒸馏水洗。(1、洗去多余的乙醇;2、润湿样本,使染液易着色)
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