当前位置：江莱生物-中国老牌试剂供应商>>技术文章>>白带样本前处理
①、取回的样本如宫颈刮片棉签，用2～3ml生理盐水冲洗，可静止30分钟后去掉上层清液，留下层沉淀，加入等体积生理盐水，取样，涂片，也可以离心去上清进行操作；
②、离心之前先将离心机中的离心管套全部取出，先平衡托盘天平(保证指针位于正中)，选取相近重量的离心管套，放入样本管，用生理盐水配平；
③、将平衡好的离心管套对称放置，(内圈6孔，外圈6孔，一般先放内孔)，一般500转/分，离心3～5分钟即可，如果需要将所有细胞沉淀，可以3000～3500转/分，离心5分钟；本实验500转/分，离心3分钟；
④、离心之后吸去上清，可以留少许上清，混匀后直接取样进行涂片，或者吸去全部上清，再加入等量的生理盐水，混匀后取样涂片；
⑤、取样3～5ul，用枪头进行涂片，涂成1cm2面积的圆形，贴上标签(编号并注明信息，同时可区别正反面，防止将样本擦掉，)；
⑥、待样本基本干透之后，可白片进行镜检，观察样本是否干净，涂片是否均匀，（如粘液依旧较多，可以重复生理盐水冲洗和离心、去上清步骤）
⑦、用95%乙醇固定涂片2～3分钟，蒸馏水洗。（1、洗去多余的乙醇；2、润湿样本，使染液易着色）
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