ELISA的方法检测多肽与小鼠血管紧张素原(aGT)测定试剂盒的结合情况。准备将合成多肽(其长度大约为8～10个氨基酸）包被于ELISA板，再加入小鼠血管紧张素原(aGT)测定试剂盒,一抗、酶标二抗。请问在包被的过程中应采取哪种包被缓冲液？固相载体是否要特殊处理？封闭液用BSA可以吗？

1、多肽抗原的包被一般需先使其与无关蛋白质如牛血清白蛋白质（BSA）等偶联，借助于偶联物与固相载体的吸附，间接地结合到固相载体表面。

2、小鼠血管紧张素原(aGT)测定试剂盒包被用抗原或抗体的浓度，包被的温度和时间，包被液的pH等应根据试验的特点和材料的性质而选定。抗体和蛋白质抗原一般采用pH9.6的碳酸盐缓冲液作为稀释液，也有用pH7.2的磷酸盐缓冲液及pH7~8的Tris-HCL缓冲液作为稀释液的。通常在ELISA板孔中加入包被液后，在4-8℃冰箱中放置过夜，37℃中保温2小时被认为具有同等的包被效果。

3、zui常用的封闭剂是0.05%-0.5%的牛血清白蛋白，小鼠血管紧张素原(aGT)测定试剂盒也有用10%的小牛血清或1%明胶作为封闭剂的。脱脂奶粉也是一种良好的封闭剂，其zui大的特点是价廉，可以高浓度使用（5%）。

显色终止后出现黑色絮状沉淀可能为包被板上的蛋白，酶标记物，检测物，封闭液中蛋白变性以及显色反应TMB过量，不溶，沉淀出来。

小鼠血管紧张素原(aGT)测定试剂盒其它优势产品如下:klB288Hu 人ATP结合盒转运蛋白G1(ABCG1)ELISA试剂盒 ELISA Kit for ATP Binding Cassette Transporter G1 (ABCG1)  Assay range：30 ng/L -1149 ng/L
klB290Hu 人V-Myc骨髓细胞瘤病毒癌基因同源物(MYC)ELISA试剂盒 ELISA Kit for V-Myc Myelocytomatosis Viral Oncogene Homolog (MYC)  Assay range：30 ng/L -1150 ng/L
klB291Hu 人V-Fos-FBJ骨肉瘤病毒癌基因同源物(FOS)ELISA试剂盒 ELISA Kit for V-Fos FBJ Murine Osteosarcoma Viral Oncogene Homolog (FOS)  Assay range：30 ng/L -1151 ng/L
klC921Hu 人血小板衍生生长因子B(PDGFB)ELISA试剂盒 ELISA Kit for Plaet Derived Growth Factor Subunit B (PDGFB)  Assay range：30 ng/L -1152 ng/L
klA728Hu 人环加氧酶1(PTGS1)ELISA试剂盒 ELISA Kit for Prostaglandin Endoperoxide Synthase 1 (PTGS1)  Assay range：30 ng/L -1153 ng/L
klA646Hu 人骨形成蛋白6(BMP6)ELISA试剂盒 ELISA Kit for Bone Morphogenetic Protein 6 (BMP6)  Assay range：30 ng/L -1154 ng/L
klA651Hu 人透明质酸结合蛋白1(HABP1)ELISA试剂盒 ELISA Kit for Hyaluronan Binding Protein 1 (HABP1)  Assay range：30 ng/L -1155 ng/L
klA687Hu 人钙结合蛋白(CR)ELISA试剂盒 ELISA Kit for Calretinin (CR)  Assay range：30 ng/L -1156 ng/L
klB298Hu 人肾上腺素能受体α1A(ADRα1A)ELISA试剂盒 ELISA Kit for Adrenergic Reklptor Alpha 1A (ADRa1A)  Assay range：30 ng/L -1157 ng/L
klB299Hu 人多巴胺受体D1(DRD1)ELISA试剂盒 ELISA Kit for Dopamine Reklptor D1 (DRD1)  Assay range：30 ng/L -1158 ng/L
klB301Ge 人不对称二甲基*(ADMA)ELISA试剂盒 ELISA Kit for Asymmetrical Dimethylarginine (ADMA)  Assay range：30 ng/L -1159 ng/L
klB302Hu 人野鼠色基因相关蛋白(AGRP)ELISA试剂盒 ELISA Kit for Agouti Related Protein (AGRP)  Assay range：30 ng/L -1160 ng/L
klB303Hu 人集聚蛋白(AGRN)ELISA试剂盒 ELISA Kit for Agrin (AGRN)  Assay range：30 ng/L -1161 ng/L
klB304Hu 人补体C5转化酶(C5c)ELISA试剂盒 ELISA Kit for Complement C5 Convertase (C5c)  Assay range：30 ng/L -1162 ng/L
klB305Hu 人补体成分4b(C4b)ELISA试剂盒 ELISA Kit for Complement Component 4b (C4b)  Assay range：30 ng/L -1163 ng/L
klB309Hu 人多效生长因子(PTN)ELISA试剂盒 ELISA Kit for Pleiotrophin (PTN)  Assay range：30 ng/L -1164 ng/L
klB310Hu 人弹性蛋白酶4(ELA4)ELISA试剂盒 ELISA Kit for Elastase 4 (ELA4)  Assay range：30 ng/L -1165 ng/L
klB311Hu 人阿黑皮素原(POMC)ELISA试剂盒 ELISA Kit for Proopiomelanocortin (POMC)  Assay range：30 ng/L -1166 ng/L
klB312Hu 人分泌性白细胞蛋白酶抑制因子(SLPI)ELISA试剂盒 ELISA Kit for Secretory Leukocyte Peptidase Inhibitor (SLPI)  Assay range：30 ng/L -1167 ng/L
klB314Hu 人*还原酶(GR)ELISA试剂盒 ELISA Kit for Glutathione Reductase (GR)  Assay range：30 ng/L -1168 ng/L
klB316Hu 人前胶原C端蛋白酶增强子1(PCPE1)ELISA试剂盒 ELISA Kit for Procollagen C Proteinase Enhanklr 1 (PCPE1)  Assay range：30 ng/L -1169 ng/L
klB317Hu 人穿孔素1(PRF1)ELISA试剂盒 ELISA Kit for Perforin 1 (PRF1)  Assay range：30 ng/L -1170 ng/L
klB319Hu 人微管关联蛋白4(MAP4)ELISA试剂盒 ELISA Kit for Microtubule Associated Protein 4 (MAP4)  Assay range：30 ng/L -1171 ng/L
klB320Hu 人胸腺基质淋巴细胞生成素(TSLP)ELISA试剂盒 ELISA Kit for Thymic Stromal Lymphopoietin (TSLP)  Assay range：30 ng/L -1172 ng/L
klA686Hu 人Ⅱ型胶原交联羧基端肽(CTXⅡ)ELISA试剂盒 ELISA Kit for Cross Linked C-opeptide Of Type II Collagen (CTXII)  Assay range：30 ng/L -1173 ng/L
klB323Hu 人钙调磷酸酶(CaN)ELISA试剂盒 ELISA Kit for Calcineurin (CaN)  Assay range：30 ng/L -1174 ng/L
klB326Hu 人神经丝蛋白3(NEF3)ELISA试剂盒 ELISA Kit for Neurofilament 3 (NEF3)  Assay range：30 ng/L -1175 ng/L
klB327Hu 人生长关联蛋白43(GAP43)ELISA试剂盒 ELISA Kit for Growth Associated Protein 43 (GAP43)  Assay range：30 ng/L -1176 ng/L
klB329Hu 人微管关联蛋白2(MAP2)ELISA试剂盒 ELISA Kit for Microtubule Associated Protein 2 (MAP2)  Assay range：30 ng/L -1177 ng/L
klB330Hu 人黑色素瘤关联ME20(ME20M)ELISA试剂盒 ELISA Kit for Melanoma Associated ME20 (ME20M)  Assay range：30 ng/L -1178 ng/L
klB331Hu 人套膜蛋白(iNV)ELISA试剂盒 ELISA Kit for Involucrin (iNV)  Assay range：30 ng/L -1179 ng/L
klB332Hu 人*脱羧酶(ODC)ELISA试剂盒 ELISA Kit for Ornithine Decarboxylase (ODC)  Assay range：30 ng/L -1180 ng/L
klB337Hu 人弹性蛋白(ELN)ELISA试剂盒 ELISA Kit for Elastin (ELN)  Assay range：30 ng/L -1181 ng/L
klB353Ge 人晚期糖基化终末产物(AGE)ELISA试剂盒 ELISA Kit for Advankld Glycation End Product (AGE)  Assay range：30 ng/L -1182 ng/L
klB354Hu 人芳香烃受体(AhR)ELISA试剂盒 ELISA Kit for Aryl Hydrocarbon Reklptor (AhR)  Assay range：30 ng/L -1183 ng/L
klB355Hu 人花生四烯酸-5-脂加氧酶(ALOX5)ELISA试剂盒 ELISA Kit for Arachidonate-5-Lipoxygenase (ALOX5)  Assay range：30 ng/L -1184 ng/L
klB357Hu 人细胞外信号调节激酶1(ERK1)ELISA试剂盒 ELISA Kit for Extraklllular Signal Regulated Kinase 1 (ERK1)  Assay range：30 ng/L -1185 ng/L
klB358Hu 人凋亡信号调节激酶I(ASK1)ELISA试剂盒 ELISA Kit for Apoptosis Signal Regulating Kinase 1 (ASK1)  Assay range：30 ng/L -1186 ng/L
klB360Hu 人酸性神经鞘磷脂酶(ASM)ELISA试剂盒 ELISA Kit for Acid Sphingomyelinase (ASM)  Assay range：30 ng/L -1187 ng/L
klB361Hu 人毛细血管扩张性共济失调突变因子(ATM)ELISA试剂盒 ELISA Kit for Ataxia angiectasia Mutated (ATM)  Assay range：30 ng/L -1188 ng/L