ELISA的方法检测多肽与小鼠胰岛素(INS)测定试剂盒的结合情况。准备将合成多肽(其长度大约为8～10个氨基酸）包被于ELISA板，再加入小鼠胰岛素(INS)测定试剂盒,一抗、酶标二抗。请问在包被的过程中应采取哪种包被缓冲液？固相载体是否要特殊处理？封闭液用BSA可以吗？

1、多肽抗原的包被一般需先使其与无关蛋白质如牛血清白蛋白质（BSA）等偶联，借助于偶联物与固相载体的吸附，间接地结合到固相载体表面。

2、小鼠胰岛素(INS)测定试剂盒包被用抗原或抗体的浓度，包被的温度和时间，包被液的pH等应根据试验的特点和材料的性质而选定。抗体和蛋白质抗原一般采用pH9.6的碳酸盐缓冲液作为稀释液，也有用pH7.2的磷酸盐缓冲液及pH7~8的Tris-HCL缓冲液作为稀释液的。通常在ELISA板孔中加入包被液后，在4-8℃冰箱中放置过夜，37℃中保温2小时被认为具有同等的包被效果。

3、zui常用的封闭剂是0.05%-0.5%的牛血清白蛋白，小鼠胰岛素(INS)测定试剂盒也有用10%的小牛血清或1%明胶作为封闭剂的。脱脂奶粉也是一种良好的封闭剂，其zui大的特点是价廉，可以高浓度使用（5%）。

显色终止后出现黑色絮状沉淀可能为包被板上的蛋白，酶标记物，检测物，封闭液中蛋白变性以及显色反应TMB过量，不溶，沉淀出来。

小鼠胰岛素(INS)测定试剂盒其它优势产品如下:

klB769Hu 人S100钙结合蛋白A6(S100A6)ELISA试剂盒 ELISA Kit for S100 Calcium Binding Protein A6 (S100A6)  Assay range：30 ng/L -1485 ng/L
klB773Hu 人角质转*酶(TGM1)ELISA试剂盒 ELISA Kit for Transglutaminase 1, Keratinocyte (TGM1)  Assay range：30 ng/L -1486 ng/L
klB780Hu 人类粘蛋白2(ORM2)ELISA试剂盒 ELISA Kit for Orosomucoid 2 (ORM2)  Assay range：30 ng/L -1487 ng/L
klB781Hu 人磷脂爬行酶1(PLSCR1)ELISA试剂盒 ELISA Kit for Phospholipid Scramblase 1 (PLSCR1)  Assay range：30 ng/L -1488 ng/L
klB785Hu 人脑啡肽酶(NEP)ELISA试剂盒 ELISA Kit for Neprilysin (NEP)  Assay range：30 ng/L -1489 ng/L
klB792Hu 人S100钙结合蛋白A8(S100A8)ELISA试剂盒 ELISA Kit for S100 Calcium Binding Protein A8 (S100A8)  Assay range：30 ng/L -1490 ng/L
klB793Hu 人世间S100钙结合蛋白A9(S100A9)ELISA试剂盒 ELISA Kit for S100 Calcium Binding Protein A9 (S100A9)  Assay range：30 ng/L -1491 ng/L
klB795Hu 人血清淀粉样蛋白A2(SAA2)ELISA试剂盒 ELISA Kit for Serum Amyloid A2 (SAA2)  Assay range：30 ng/L -1492 ng/L
klB796Hu 人信号传导转录激活因子2(STAT2)ELISA试剂盒 ELISA Kit for Signal Transduklr And Activator Of Transcription 2 (STAT2)  Assay range：30 ng/L -1493 ng/L
klB801Hu 人*原(PK)ELISA试剂盒 ELISA Kit for Prekallikrein (PK)  Assay range：30 ng/L -1494 ng/L
klB805Hu 人高分子量激肽原(HMWK)ELISA试剂盒 ELISA Kit for High Molecular Weight Kininogen (HMWK)  Assay range：30 ng/L -1495 ng/L
klB808Hu 人粘蛋白7(MUC7)ELISA试剂盒 ELISA Kit for Mucin 7, Secreted (MUC7)  Assay range：30 ng/L -1496 ng/L
klA680Hu 人棕榈酰化膜蛋白2(MPP2)ELISA试剂盒 ELISA Kit for Membrane Protein, Palmitoylated 2 (MPP2)  Assay range：30 ng/L -1497 ng/L
klA681Hu 人乙醇脱氢酶1(ADH1)ELISA试剂盒 ELISA Kit for Alcohol Dehydrogenase 1 (ADH1)  Assay range：30 ng/L -1498 ng/L
klB810Hu 人*结合蛋白(DBP)ELISA试剂盒 ELISA Kit for Vitamin D Binding Protein (DBP)  Assay range：30 ng/L -1499 ng/L
klB817Hu 人多功能蛋白聚糖(VS)ELISA试剂盒 ELISA Kit for Versican (VS)  Assay range：30 ng/L -1500 ng/L
klB818Hu 人血管内皮生长因子受体1(VEGFR1)ELISA试剂盒 ELISA Kit for Vascular Endothelial Growth Factor Reklptor 1 (VEGFR1)  Assay range：30 ng/L -1501 ng/L
klB819Hu 人再生胰岛衍生蛋白4(REG4)ELISA试剂盒 ELISA Kit for Regenerating Islet Derived Protein 4 (REG4)  Assay range：30 ng/L -1502 ng/L
klB822Hu 人角蛋白17(KRT17)ELISA试剂盒 ELISA Kit for Keratin 17 (KRT17)  Assay range：30 ng/L -1503 ng/L
klB827Hu 人轴突生长诱向因子1(Ntn1)ELISA试剂盒 ELISA Kit for Netrin 1 (Ntn1)  Assay range：30 ng/L -1504 ng/L
klB835Hu 人轴突生长诱向因子4(Ntn4)ELISA试剂盒 ELISA Kit for Netrin 4 (Ntn4)  Assay range：30 ng/L -1505 ng/L
klB967Hu 人载脂蛋白A4(APOA4)ELISA试剂盒 ELISA Kit for Apolipoprotein A4 (APOA4)  Assay range：30 ng/L -1506 ng/L
klB829Hu 人解整合素金属蛋白酶9(ADAM9)ELISA试剂盒 ELISA Kit for A Disintegrin And Metalloprotease 9 (ADAM9)  Assay range：30 ng/L -1507 ng/L
klB830Hu 人组织转*酶(TGM2)ELISA试剂盒 ELISA Kit for Transglutaminase 2, Tissue (TGM2)  Assay range：30 ng/L -1508 ng/L
klB831Hu 人音猬因子(SHH)ELISA试剂盒 ELISA Kit for Hedgehog Homolog, Sonic (SHH)  Assay range：30 ng/L -1509 ng/L
klA111Hu 人白介素12(IL12)ELISA试剂盒 ELISA Kit for Interleukin 12 (IL12)  Assay range：30 ng/L -1510 ng/L
klB833Hu 人补体因子D(CFD)ELISA试剂盒 ELISA Kit for Complement Factor D (CFD)  Assay range：30 ng/L -1511 ng/L
klB834Hu 人*Ⅺ(F11)ELISA试剂盒 ELISA Kit for Coagulation Factor XI (F11)  Assay range：30 ng/L -1512 ng/L
klB839Hu 人纽蛋白(VCL)ELISA试剂盒 ELISA Kit for Vinculin (VCL)  Assay range：30 ng/L -1513 ng/L
klB848Hu 人核因子κB抑制因子α(IκBα)ELISA试剂盒 ELISA Kit for Inhibitory Subunit Of NF Kappa B Alpha (IkBa)  Assay range：30 ng/L -1514 ng/L
klB850Hu 人β-1,4-半乳糖转移酶1(β4GALT1)ELISA试剂盒 ELISA Kit for Beta-1,4-Galactosyltransferase 1 (b4GALT1)  Assay range：30 ng/L -1515 ng/L
klA143Hu 人血管内皮生长因子A(VEGFA)ELISA试剂盒 ELISA Kit for Vascular Endothelial Growth Factor A (VEGFA)  Assay range：30 ng/L -1516 ng/L
klB852Hu 人含Rho关联卷曲螺旋蛋白激酶2(Rock2)ELISA试剂盒 ELISA Kit for Rho Associated Coiled Coil Containing Protein Kinase 2 (Rock2)  Assay range：30 ng/L -1517 ng/L
klB859Hu 人凝集素样氧化低密度脂蛋白受体1(LOX1)ELISA试剂盒 ELISA Kit for Lectin Like Oxidized Low Density Lipoprotein Reklptor 1 (LOX1)  Assay range：30 ng/L -1518 ng/L
klB860Hu 人胎球蛋白B(FETUB)ELISA试剂盒 ELISA Kit for Fetuin B (FETUB)  Assay range：30 ng/L -1519 ng/L
klB824Hu 人核因子κB(NFκB)ELISA试剂盒 ELISA Kit for Nuclear Factor Kappa B (NFkB)  Assay range：30 ng/L -1520 ng/L
klB856Hu 人Ⅰ类主要组织相容性复合体G(MHCG)ELISA试剂盒 ELISA Kit for Major Histocompatibility Complex Class I G (MHCG)  Assay range：30 ng/L -1521 ng/L
klB866Hu 人神经调节素1(NRG1)ELISA试剂盒 ELISA Kit for Neuregulin 1 (NRG1)  Assay range：30 ng/L -1522 ng/L
klB867Hu 人表皮生长因子受体2(EGFR2)ELISA试剂盒 ELISA Kit for Epidermal Growth Factor Reklptor 2 (EGFR2)  Assay range：30 ng/L -1523 ng/L