人羊膜上皮细胞 7110以高品质、低价格、快供货、更优服务为广大科研者提供更为完善的产品供应渠道和售后服务。
培养条件:DMEM+10%FBS;37℃,5%CO2
规格:70%密度的25cm2培养瓶的细胞一瓶
细胞传代:1:3 传代
细胞来源:ATCC、sciencell、ICLC
货期:1-2周
产品运输方式:干冰或者活体细胞运输,本公司保证细胞运输中的存活率细胞融合与杂交瘤人羊膜上皮细胞 7110的选择性培养:
细胞融合的程序已报道的有很多种,这里介绍的是作者所在实验室常用的一种。
A、 将1×108脾细胞与2×107-5×107骨髓瘤细胞SP2/0-Ag14混合于一支50ml融合管中,补加不*培养基至30ml,充分混匀。
B、 1000r/min离心5-10分钟,将上清尽量吸净。
C、 在手掌上轻击融合管底,使沉淀细胞松散均匀;置40℃水浴中预热。
D、 人羊膜上皮细胞 7110用1ml吸管在1分钟左右(zui好时间为45秒)加预热至40℃的50% PEG(PH 8.0)1ml,边加边轻轻搅拌。
E、 用10ml吸管在90秒内加20-30ml预热至37℃的不*培养基;20-37℃静置10分钟。
F、 1000r/min 5分钟;弃去上清。
G、 加入5ml HAT培养基,轻轻吹吸沉淀细胞,使其悬浮并混匀,然后补加含腹腔巨噬细胞的HAT培养基至80-100ml。
H、 分装96孔细胞培养板,每孔0.10-0.15ml;分装24孔板,每孔1.0-1.5ml;然后将培养板置37℃,6% CO2培养箱内培养。
I、 5天后用HAT培养基换出1/2培养基。
J、 7-10天后用HT培养基换出HAT培养基;(第14天后可用普通*培养基)。
L、 人羊膜上皮细胞 7110经常观察杂交瘤细胞生长情况,待其长至孔底面积1/10以上时吸出上清供抗体检测。
kl1632 间充质干细胞
kl1633 HAMSC 人羊膜间充质基质细胞 Human Amniotic Mesenchymal Stromal Cells 5 x 10^5 cells/vial
kl1634 HCMSC 人绒毛膜间充质基质细胞 Human Chorionic Mesenchymal Stromal Cells 5 x 10^5 cells/vial
kl1635 HMSC-bm 人间充质干细胞--骨髓 Human Mesenchymal Stem Cells-bone marrow 5 x 10^5 cells/vial
kl1636 HMSC-ad 人间充质干细胞--脂肪 Human Mesenchymal Stem Cells-adipose 5 x 10^5 cells/vial
kl1637 HMSC-hp 人间充质干细胞--肝脏 Human Mesenchymal Stem Cells-hepatic 5 x 10^5 cells/vial
kl1638 HUMSC 人脐带间充质干细胞 Human Umbilical Mesenchymal Stem Cells 5 x 10^5 cells/vial
kl1639 HPMSC 人肺间充质干细胞 Human Pulmonary Mesenchymal Stem Cells 5 x 10^5 cells/vial
kl1640 HVMSC 人脊椎间充质干细胞 Human Vertebral Mesenchymal Stem Cells 5 x 10^5 cells/vial
kl1641 MSCM 间充质干细胞培养基 Mesenchymal Stem Cell Medium 500 ml
kl1642 MODM-500 间充质干细胞-成骨细胞分化培养基 Mesenchymal Stem Cell Oseogenic Differentiation Medium 500 ml
kl1643 MODM-250 Mesenchymal Stem Cell Oseogenic Differentiation Medium 250 ml
kl1644 MODM-b 间充质干细胞-成骨细胞分化培养基-基础 Mesenchymal Stem Cell Osteogenic Differentiation Medium-basal 500ml
kl1645 MODM-b-prf 间充质干细胞-成骨细胞分化培养基-无酚红 Mesenchymal Stem Cell Osteogenic Differentiation Medium-basal-phenol red free 500ml
kl1646 MADM-500 间充质干细胞-脂肪细胞分化培养基 Mesenchymal Stem Cell Adipogenic Differentiation Medium 500 ml
kl1647 MADM-250 Mesenchymal Stem Cell Adipogenic Differentiation Medium 250 ml
kl1648 MADM-b 间充质干细胞-脂肪细胞分化培养基-基础 Mesenchymal Stem Cell Adipogenic Differentiation Medium-basal 500 ml
kl1649 MADM-b-prf 间充质干细胞-脂肪细胞分化培养基-无酚红 Mesenchymal Stem Cell Adipogenic Differentiation Medium-basal-phenol red free 500ml
kl1650 MCDM 间充质干细胞-软骨分化培养基 Mesenchymal Stem Cell Chondrogenic Differentiation Medium 500ml
kl1651 Ared 茜素红S染色试剂盒 Alizarin Red S Staining Kit 100 ml
kl1652 Ored 油红O染色试剂盒 Oil Red O Staining Kit 100 ml
kl1653 PCR 人间质干细胞成脂肪细胞检测试剂盒 Human Mesenchymal Stem Cell Adipogenesis Detection Kit 50 reactions
kl1654 HMSC-C PCR 人间充质干细胞软骨形成检测试剂盒 Human Mesenchymal Stem Cell Chondrogenesis Detection Kit 50 reactions
kl1655 SafraninO 番红O染色试剂盒 Safranin O Staining Kit 100 reactions
kl1656 ABlue 阿利新蓝染色试剂盒 Alcian Blue Staining Kit 100 reactions
kl1657 重编程
kl1658 HDF-f 人真皮成纤维细胞--胎儿 Human Dermal Fibroblasts-fetal 5 x 10^5 cells/vial
kl1659 HDF-n 人真皮成纤维细胞--新生 Human Dermal Fibroblasts-neonate 5 x 10^5 cells/vial
kl1660 HDF-a 人真皮成纤维细胞--成人 Human Dermal Fibroblasts-adult 5 x 10^5 cells/vial
kl1661 HEK-n 人表皮角质细胞--新生 Human Epidermal Keratinocytes-neonatal 5 x 10^5 cells/vial
kl1662 AEpiCM 肺泡上皮细胞培养基 Alveolar Epithelial Cell Medium 500 ml
kl1663 KM 角质细胞培养基 Keratinocyte Medium 500 ml
kl1664 干细胞检测试剂盒
kl1665 HGL PCR 人胚层检测试剂盒 Human Germ Layer Detection Kit 50 reactions
kl1666 HSC PCR 人干细胞多能性检测试剂盒 Human Stem Cell Pluripotency Detection Kit 50 reactions
kl1667 ALP 碱性磷酸酶活性测定试剂盒 Alkaline Phosphatase Activity Assay 500 tests
kl1668 ALPr 碱性磷酸酶染色检测(红色) Alkaline Phosphatase Staining Assay (Red) 500 tests
kl1669
kl1670 Cell Transfection Kits
kl1671 EndoFectagen. 内皮细胞转染试剂盒 Endothelial Cell Transfection Kit 250 Transfections
kl1672 FibroFectagen. 成纤维细胞转染试剂 Fibroblast Transfection Kit 200 Transfections
kl1673 EpiFectagen. 上皮细胞转染试剂盒 Epithelial Cell Transfection Kit 100 Transfections
kl1674 MesenFectagen. 间充质干细胞转染试剂盒 Mesenchymal Stem Cell Transfection Kit 250 Transfections
kl1675 AstroFectagen. 星形胶质细胞转染试剂盒 Astrocyte Transfection Kit 250 Transfections
kl1676 SMCFectagen. 平滑肌细胞转染试剂盒 Smooth Muscle Cell Transfection Kit 250 Transfections
kl1677 KeratoFectagen. 角质细胞转染试剂盒 Keratinocyte Transfection Kit 200 Transfections
kl1678 EpiFectagen II. 上皮细胞转染试剂盒 Epithelial Cell Transfection Kit 250 Transfections
kl1679
kl1680 Cell Based Assay
kl1681 血管生成检测试剂盒
kl1682 IVTFA 体外管腔形成分析试剂盒 In Vitro Tube Fomation Assay (Angiogenesis Assay) 50 tests/96 well plate
kl1683 细胞粘附试剂盒
kl1684 Col I型胶原细胞粘附检测试剂盒 Collagen I Cell Adhesion Assay 48 tests/48 well plate
kl1685 Fibro 纤维粘连细胞粘附检测试剂盒 Fibronectin Cell Adhesion Assay 48 tests/48 well plate
kl1686 C3DGK 胶原I-3D凝胶试剂盒 Collagen I-3D Gelling Kit 100 mg
kl1687 CCCSCK 胶原I-细胞培养表面包被试剂盒 Collage I-Cell Culture Surface Coating Kit 10 mg
kl1688 细胞活力与凋亡检测试剂盒
kl1689 MTT MTT细胞活力和增殖检测试剂盒 MTT Cell Viability and Proliferation Assay 1000 tests/96 well plate
kl1690 WST WST-1细胞活力和增殖检测试剂盒 WST-1 Cell Viability and Proliferation Assay 1000 tests/96 well plate
kl1691 CSA 细胞衰老检测试剂盒 Cell Senescence Assay 50 tests/53 mm plate
kl1692 LDH LDH细胞毒性检测试剂盒 LDH Cytotoxicity Assay 500 tests/96 well plate
kl1693 TUNEL TUNEL色标法细胞凋亡检测试剂盒 Colorimetric TUNEL Apoptosis Assay 50 tests/96 well plate
kl1694 CSK 活/死细胞检测试剂盒 Live/Dead Cell Staining Kit 1000 tests/96 well plate