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α2-巨球蛋白(α2M)多克隆抗体
Polyclonal Antibody to Alpha-2-Macroglobulin (a2M)
FOR IN VITRO AND RESEARCH USE ONLY, NOT FOR USE IN CLINICAL DIAGNOSTIC PROCEDURES!
Organism species | Oryctolagus cuniculus (Rabbit) |
Product No. | ybB017Rb51 |
Clonality | Polyclonal |
Host | Guinea Pig |
Immunoglobulin Type | IgG |
Purification | Affinity Chromatography |
Applications | WB, ICC, IHC-P, IHC-F, ELISA |
Concentration | 200ug/ml |
UOM | 100ug |
Conjugate | No Conjugate |
α2-巨球蛋白(α2M)多克隆抗体 ANTIBODY SPECIFITY
The antibody is a cavia polyclonal antibody raised against a2M. It has been selected for its ability to recognize a2M in immunohistochemical staining and western blotting.
APPLICATIONS
Western blotting: 1:50-400
Immunocytochemistry in formalin fixed cells: 1:50-500
Immunohistochemistry in formalin fixed frozen section: 1:50-500
Immunohistochemistry in paraffin section: 1:10-100
Enzyme-linked Immunosorbent Assay: 1:100-200
Optimal working dilutions must be determined by end user.
α2-巨球蛋白(α2M)多克隆抗体 CONTENTS
Form & Buffer: Supplied as solution form in PBS, pH7.4,
containing 0.02% NaN3, 50% glycerol.
IHC Images
α2-巨球蛋白(α2M)多克隆抗体
QUALITY CONTROL
Content: The quality control contains recombinant a2M (Leu772~Arg1056) disposed in loading buffer.
Usage: 10uL per well when 3,3'-Diaminobenzidine(DAB) as the substrate.
5uL per well when used in enhanced chemilumescent (ECL).
Note: The quality control is specifically manufactured as the positive control. Not used for other purposes.
Loading Buffer: 100mM Tris(pH8.8), 2% SDS, 200mM NaCl, 50% glycerol, BPB 0.01%, NaN3 0.02%.
STORAGE
Store at 4oC for frequent use. Stored at -20oC in a manual defrost freezer for one year without detectable loss of activity. Avoid repeated freeze-thaw cycles.
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