中文名称：人Smad7ELISA试剂盒
英文名称：Human Smad7 ELISA Kit
规 格：96T/Kit (8*12 strips) 48T/Kit (8*6 strips）
保存和有效期：2-8℃/6个月
运输条件：低温运输，用干冰或者冰袋低温运输。
可测种属：人、大小鼠、豚鼠、兔子、猪、犬、猴、马、、、、、、
可测标本：血清、血浆、细胞上清液、尿液、体液、灌洗液、、、、、、、
定义：一种酶联免疫技术，用于检测被于固相板孔中的待测抗原（或抗体）。即用酶标记抗体，并将已知抗原或抗体吸附在固相载体表面，使抗原抗体反应在固相载体表面进行，用洗涤法将液相中的游离成分洗除，zui后通过酶作用于底物后显色来判断结果。
人Smad7ELISA试剂盒专一性强、灵敏度高、样品易保存。
应用学科：免疫学（一级学科）；应用免疫（二级学科）；免疫学检测和诊断（二级学科）
人Smad7ELISA试剂盒必须遵守以下3个核心原理：
1）抗原或抗体能吸附于固相载体表面，并保持其免疫学活性；
2）抗原或抗体可通过共价键与酶连接形成酶结合物，而此种酶结合物仍能保持其免疫学和酶学活性；
3）酶结合物与相应抗原或抗体结合后，可根据加入底物的颜色反应来判定是否有免疫反应的存在，而且颜色反应的深浅是与标本中相应抗原或抗体的量成正比例的，可根据已知浓度的抗原或抗体的颜色反应的深浅绘制标准曲线并依此计算出未知样品中抗体或抗原的浓度。在ELISA中酶起到关键的作用，常用于ELISA法的酶有辣根过氧化物酶，碱性磷酸酯酶等，其中以辣根过氧化物酶为多，人Smad7ELISA试剂盒酶结合物是酶与抗体或抗原，半抗原在交联剂作用下联结的产物，它不仅具有抗体抗原特异的免疫反应，还能催化酶促反应，显示出生物放大作用。
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