产品编号 yb-4269R
英文名称 Anti-CDKN2B/p15 INK4b抗体
中文名称 细胞周期蛋白依赖性激酶抑制剂2B抗体
别    名 CDK inhibitory protein; CDK4B Inhibitor; CDKN2B; Cyclin dependent kinase 4 inhibitor B; Cyclin Dependent Kinase Inhibitor 2B; Cyclin dependent kinase inhibitor 2B p15 inhibits CDK4; Cyclin-dependent kinases 4 and 6 binding protein; INK4B; MTS 2; MTS2; Multiple Tumor Supressor 2; p14 CDK inhibitor; p14 INK4b; P15; p15 CDK inhibitor; p15 inhibits CDK4; p15 INK4b; P15(INK4B); p15INK4B; TP 15; TP15.
Anti-CDKN2B/p15 INK4b抗体    
说 明 书 0.1ml  0.2ml  
研究领域 肿瘤  细胞生物  免疫学  信号转导  干细胞  细胞周期蛋白  
抗体来源 Rabbit
克隆类型 Polyclonal
交叉反应 Human, Mouse, Rat, Chicken, Dog, Cow, Rabbit, 
产品应用 WB=1:100-500 ELISA=1:500-1000 IHC-P=1:100-500 IHC-F=1:100-500 IF=1:100-500 （石蜡切片需做抗原修复） 
not yet tested in other applications.
optimal dilutions/concentrations should be determined by the end user.
分 子 量 15kDa
细胞定位 细胞浆 
性    状 Lyophilized or Liquid
浓    度 1mg/1ml
免 疫 原 KLH conjugated synthetic peptide derived from human CDKN2B
亚    型 IgG
纯化方法 affinity purified by Protein A
储 存 液 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.
保存条件 Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C.

PubMed PubMed
产品介绍 background:
p15 INK4b / MTS 2 (Mitotic Inhibitor / Suppressor Protein) is a specific inhibitor of cdk 4 / cdk 6 and is periodically expressed during the cell cycle. Reportedly, p15 INK4b expression is induced approximay 30 fold in human keratinocytes by treatment with TGF beta, suggesting that p15 INK4b may act as an effector of TGF beta mediated cell cycle arrest. p15 INK4b is mapped to chromosome 9p21, a region of frequent loss in a wide variety of cancers.

Function:
Interacts strongly with CDK4 and CDK6. Potent inhibitor. Potential effector of TGF-beta induced cell cycle arrest.

Subunit:
Heterodimer of CDKN2B with CDK4 or CDK6. Isoform 2 does not interact with CDK4 nor CDK6.

Subcellular Location:
Cytoplasm. Note=Also found in the nucleus.

Tissue Specificity:
Isoform 2 is expressed in normal (keratinocytes, fibroblasts) and tumor cell lines.

Similarity:
Belongs to the CDKN2 cyclin-dependent kinase inhibitor family.
Contains 4 ANK repeats.


Important Note:
This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.