6-磷酸葡萄糖脱氢酶(大杆菌)6-Phosphogluconate dehydrogenase (E. coli) 150U 爱尔兰 型号:JKY/E-PGDHEC
型号:JKY/E-PGDHEC
库号：M314678
外观：悬浮液
在水中的溶解性：易溶。
pH值：6.0
气味：无
状态：液体
保质期：在冰箱5年或以上,4度,2年以上.
成分：
名称 比例
6磷酸葡萄糖脱氢酶 1％
硫酸铵 45％
叠氮钠 0.02％
水 54％
规格:
150U,21.4 U/mg （25oC, pH 7.6）
EC 1.1.1.44. Recombinant from E. coli. In 3.2 M ammonium sulphate. Specific activity: 21.4 U/mg （25oC, pH 7.6）. Contaminants: NADH oxidase = 0.00015 %, NADPH oxidase < 0.001 %.
Stable at 4oC for > 2 years.
PROPERTIES
1. ELECTROPHORETIC PURITY
- Single band on SDS-gel electrophoresis （MW ~ 52,546）
- Single major band on isoelectric focusing （pI ~ 5.3）
2. SPECIFIC ACTIVITY AND LEVEL OF OTHER ACTIVITIES
21.4 U/mg protein at pH 7.6 and 25°C.
One Unit of 6-phosphogluconate dehydrogenase （6-PGDH） is defined as the amount
of enzyme required to produce one µmole of NADPH from NADP+
under the
following assay conditions:
TEA buffer, pH 7.6 86 mM
ATP 7.5 mM
MgCl
2 8.6 mM
D-Gluconic acid 3.2 mM
NADP+ 0.9 mM
Gluconate kinase 6.0 U/mL
3. OTHER ACTIVITIES （as a percentage of 6-PGDH activity）
Enzyme measured Substrate Activity, %
6-PGDH D-gluconate 6-phosphate 100
NADH oxidase NADH 0.00015
NADPH oxidase NADPH 0.000000
4. PHYSICOCHEMICAL PROPERTIES:
Recommended conditions of use are at pH 7.6 and up to 25°C.
5. STORAGE AND USE CONDITIONS/RECOMMENDATIONS:
The enzyme is supplied as an ammonium sulphate suspension and should be stored at
4°C. Swirl to mix the enzyme suspension immediay prior to use.
Product Code E-PGDHEC
Product Name 6-Phosphogluconate Dehydrogenase
Proper Shipping Name as above
Other Names None Listed
UN Number None Allocated
DG Class None Allocated
Packing Group None Allocated
Hazchem Code None Allocated
Poisins Schedule Not Scheduled
Appearance Liquid suspension
Specific Gravity ~ 1.5
Solubility in Water Readily soluble
pH Value 6.0
Odour None
Form Liquid
Stability Stable in a refrigerator for five or more years
Ingredients Name CAS Proportion
6-Phosphogluconate Dehydrogenase 1%
Ammonium Sulphate 45%
Sodium Azide 0.02%
Water 54%
用途:科研,食品
分类:试剂,囯际直购
6-磷酸葡萄糖脱氢酶（大杆菌）6-Phosphogluconate dehydrogenase （E. coli） 150U 爱尔兰
