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厦门慧嘉生物*经营小鼠抗甲状腺过氧化物酶抗体(TPO-Ab)ELISA试剂盒 ,价格实惠,服务周到,质量有保证。欢迎广告老师来询!: :
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小鼠白细胞介素-1受体拮抗剂(IL1Ra)ELISA试剂盒 |
小鼠降钙素原(PCT)ELISA试剂盒 |
小鼠胰岛素自身抗体(IAA)ELISA试剂盒 |
小鼠白细胞介素-7(IL-7)ELISA试剂盒 |
小鼠褪黑素(MT)ELISA试剂盒 |
小鼠肾上腺髓质素(ADM)ELISA试剂盒 |
小鼠可溶性瘦素受体(sLR)ELISA试剂盒 |
小鼠β内啡肽受体(β-EPR)ELISA试剂盒 |
小鼠肝脂酶(HL)ELISA试剂盒 |
小鼠二胺氧化酶(DAO)ELISA试剂盒 |
检测范围: | Request Information |
灵敏度: | Request Information |
反应时间: | 1-3h |
所需样本体积: | 50-100ul |
检测波长: | 450 nm |
说明书: | (向客服人员索取原版说明书) |
原理 : | This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for ACHE has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any ACHE present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for ACHE is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of ACHE bound in the initial step. The color development is stopped and the intensity of the color is measured. |
特异性: | This assay has high sensitivity and excellent specificity for detection of Rat ACHE. No significant cross-reactivity or interference between Rat ACHE and analogues was observed. |
精密度: | Intra-assay Precision (Precision within an assay): CV%<8% |
样本搜集及储存: | Serum: Use a serum separator tube (SST) and allow samples to clot for two hours at room temperature or overnight at 4°C before centrifugation for 15 minutes at 1000 ×g. Remove serum and assay immediay or aliquot and store samples at -20°C or -80°C. Avoid repeated freeze-thaw cycles. |
检测步骤: | Bring all reagents and samples to room temperature before use. Centrifuge the sample again after thawing before the assay. It is recommended that all samples and standards be assayed in duplicate. |
结果计算: | Using the professional soft "Curve Exert 1.3" to make a standard curve is recommended, which can be downloaded from our web. |
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