| Synonyms | Small inducible cytokine B10, CXCL10, 10 kDa interferon-gamma-induced protein, Gamma-IP10, IP-10, chemokine （C-X-C motif） ligand 10, C7, IFI10, INP10, crg-2, mob-1, SCYB10, gIP-10. |
| Introduction | Chemokine （C-X-C motif） ligand 10 （CXCL10） is a small cytokine belonging to the CXC chemokine family that is also known as 10 kDa interferon-gamma-induced protein （?-IP10 or IP-10）. CXCL10 is secreted by several cell types in response to IFN-?. These cell types include monocytes, endothelial cells and fibroblasts. CXCL10 has been attributed to several roles, such as chemoattraction for monocytes and T cells, promotion of T cell adhesion to endothelial cells, antitumor activity, and inhibition of bone marrow colony formation and angiogenesis. The gene for CXCL10 is located on human chromosome 4 in a cluster among several other CXC chemokines. This chemokine elicits its effects by binding to the cell surface chemokine receptor CXCR3. The three-dimensional crystal structure of this chemokine has been determined under 3 different conditions to a resolution of up to 1.92A. |
| Description | IP-10 Mouse Recombinant produced in E.Coli is a single,non-glycosylated, polypeptide chain containing 77 amino acids and having a molecular mass of 8701Dalton.
The IP-10 is purified by proprietary chromatographic techniques. |
| Source | Escherichia Coli. |
| Physical Appearance | Sterile Filtered White lyophilized （freeze-dried） powder. |
| Formulation | The CXCL10 protein was lyophilized with no additives. |
| Solubility | It is recommended to reconstitute the lyophilized IP-10 in sterile 18MΩ-cm H2O not less than 100μg/ml, which can then be further diluted to other aqueous solutions. |
| Stability | Lyophilized IP-10 although stable at room temperature for 3 weeks, should be stored desiccated below -18°C. Upon reconstitution CXCL10 should be stored at 4°C between 2-7 days and for future use below -18°C. For long term storage it is recommended to add a carrier protein （0.1% HSA or BSA）.
Please prevent freeze-thaw cycles. |
| Purity | Greater than 97.0% as determined by（a） Analysis by RP-HPLC.
（b） Analysis by SDS-PAGE. |
| Amino acid sequence | The sequence of the first five N-terminal amino acids was determined and was found to be Ile-Pro-Leu-Ala-Arg. |
| Biological Activity | The Biological activity is calculated by its ability to chemoattract IL-2 activated T cells
at 0.1-10 ng/ml. |
| Protein content | Protein quantitation was carried out by two independent methods
1. UV spectroscopy at 280 nm using the absorbency value of 0.02 as the extinction coefficient for a 0.1% （1mg/ml） solution. This value is calculated by the PC GENE computer analysis program of protein sequences （InliGenetics）.
2. Analysis by RP-HPLC, using a standard solution of IP-10 as a Reference Standard. |
| Usage | Prospec's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals. |
