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EPO a Fc Human/重组人红细胞生成素- α受体/嵌合体

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EPO a Fc Human/重组人红细胞生成素- α受体/嵌合体产品编号：CYT-325
产品规格：2μg/10μg/1mg

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EPO a Fc Human/重组人红细胞生成素- α受体/嵌合体

产品编号：CYT-325
产品规格：2μg/10μg/1mg

Synonyms	EPO-?, EPO-alpha, Epoetin, EP, MGC138142.
Introduction	This gene is a member of the EPO/TPO family and encodes a secreted, glycosylated cytokine composed of four alpha helical bundles. The protein is found in the plasma and regulates red cell production by promoting erythroid differentiation and initiating hemoglobin synthesis. This protein also has neuroprotective activity against a variety of potential brain injuries and antiapoptotic functions in several tissue types.
Description	Erythropoietin-alpha Fc-Chimera Human Recombinant is produced in Chinese hamster ovary （CHO） cells by recombinant DNA technology is a dimeric, glycosilated, polypeptide chain consisting of two mature human EPO molecules linked to the Fc portion of human IgG1. The Fc component contains the CH2 domain, the CH3 domain and hinge region, but not the CH1 domain of IgG1. As a result of glycosylation, the recombinant protein migrates with an apparent molecular mass of 140 kDa in non-reducing SDS-PAGE.
Source	Chinese Hamster Ovary Cells（CHO）.
Physical Appearance	Sterile Filtered White lyophilized （freeze-dried） powder.
Formulation	Each mg of lyophilized powder contains 1x PBS pH-7.4.
Solubility	It is recommended to reconstitute the lyophilized Erythropoietin in sterile 18MΩ-cm H2O not less than 100μg/ml, which can then be further diluted to other aqueous solutions.
Stability	Lyophilized Erythropoietin-a although stable at room temperature for 3 weeks, should be stored desiccated below -18°C. Upon reconstitution EPO-alpha should be stored at 4°C between 2-7 days and for future use below -18°C. For long term storage it is recommended to add a carrier protein （0.1% HSA or BSA）. Please prevent freeze-thaw cycles.
Purity	Greater than 98.0% as determined by（a） Analysis by RP-HPLC. （b） Analysis by SDS-PAGE.
Biological Activity	The ED50 as determined by the dose-dependant stimulation of human megakaryoblastic leukemia cells is less than 2.0 ng/ml, corresponding to a Specific Activity of 5.0 x 105IU/mg.
Usage	ProSpec's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.