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重组多磷酸激酶/PPK

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更新时间:2016-05-12 05:27:49浏览次数:953次

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产品简介

重组多磷酸激酶/PPK 产品编号:ENZ-266
产品规格:50IU/100IU/200IU

详细介绍

重组多磷酸激酶/PPK

产品编号:ENZ-266
产品规格:50IU/100IU/200IU
 

Catalogue Number ENZ-266
Synonyms Polyphosphate kinase, EC 2.7.4.1, Polyphosphoric acid kinase, ATP-polyphosphate phosphotransferase.
Introduction PPK catalyzes the reversible transfer of phosphate between polyphosphate and ATP. The phosphorylation of ADP to ATP by polyphosphate kinase is by a processive mechanism; the phosphorylation occurs without release of the polymer from the enzyme prior to termination of the reaction.
Description Polyphosphate Kinase purified circa 10 fold . Free of all Polyphosphate Glucokinase activity. Native MW = 83 kD (monomer).
Source Propionibacterium shermanii.
Physical Appearance Sterile Filtered White lyophilized (freeze-dried) powder.
Formulation The protein was lyophilized from 1.54ml PPK solution containing 32.45U/ml of PPK activity, 3.87mg/ml total protein, 10mM potassium phosphate pH 6.8 and 25mM sodium polyphosphate.
Solubility The contents of the vial can be reconstituted in 1-10 ml deionized water.
Stability Lyophilized Polyphosphate kinase although stable at room temperature for 3 weeks, should be stored desiccated below -18°C. Upon reconstitution PPK should be stored at 4°C between 2-7 days and for future use below -18°C.
For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).
Please prevent freeze-thaw cycles.
Definition of Activity Units The amount of Polyphosphate kinase required to convert 1 μmole ADP to ATP per minute at pH 7.5, using polyphosphate as phosphate donor.
Specific Activity 8.39 U/mg.
Required Cofactors The reaction requires Mg2+.
Usage Prospec's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.
 
   
   
   
   
 


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