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重组乳酸脱氢酶B/LDHB

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重组乳酸脱氢酶B/LDHB产品编号：ENZ-279
产品规格：1mg/3mg/30mg

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重组乳酸脱氢酶B/LDHB

产品编号：ENZ-279
产品规格：1mg/3mg/30mg

Synonyms	Lactate Dehydrogenase, LDH.
Introduction	Lactate dehydrogenase （LDH） is an enzyme（EC1.1.1.27） present in a wide variety of organisms, including plants and animals. A tetrameric enzyme that catalyses the interconversion of pyruvateand lactatewith concomitant interconversion of NADH and NAD+. At high concentrations of pyruvate, the enzyme exhibits feedback inhibition and the rate of conversion of pyruvate to lactate is decreased. In vertebrates, genes for three different subunits （LDH-A, LDH-B and LDH-C） exist.
Description	The DNA encoding chicken LDH-B is cloned from cDNA library of chicken heart.
Source	Escherichia Coli.
Physical Appearance	Sterile lyophilized powder.
Formulation	The protein （1 mg/ml） was lyophilized with 0.1mg potassium phosphate.
Solubility	It is recommended to reconstitute the lyophilized LDH in sterile 18MΩ-cm H2O not less than 100 μg/ml, which can then be further diluted to other aqueous solutions.
Stability	Lyophilized Lactate Dehydrogenase although stable at room temperature for 3 weeks, should be stored desiccated below -18°C. Upon reconstitution LDH should be stored at 4°C between 2-7 days and for future use below -18°C. Please prevent freeze-thaw cycles.
Purity	Greater than 95.0% as determined by（a） Analysis by RP-HPLC. （b） Analysis by SDS-PAGE.
Biological Activity	The specific activity was found to be 258 U/mg protein.
Unit Definition	One unit is defined as 1umol of NAD+ production per minute under the assay conditions （25°C, pH 7.0）. Both transaminase activities include a-hydroxyglutarate dehydrogenase activity.
Usage	Prospec's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.