Mouse Anti-SOD抗体说明书，现货wb操作

FROM:abcam

Catalog Number : RS-1079R

Quantity size :0.1ml (dilute with pH 7.4 0.01 M PBS or antibody diluent )
background:
The protein encoded by this gene binds copper and zinc ions and is one of two isozymes responsible for destroying free superoxide radicals in the body. The encoded isozyme is a soluble cytoplasmic protein, acting as a homodimer to convert naturally-occuring but harmful superoxide radicals to molecular oxygen and hydrogen peroxide. The other isozyme is a mitochondrial protein. Mutations in this gene have been implicated as causes of familial amyotrophic lateral sclerosis. Rare transcript variants have been reported for this gene. [provided by RefSeq, Jul 2008]

Specificity :

–anti-SOD is a mouse Polyclonal antibody.Anti-SOD抗体说明书，现货wb操作

– specific for anti-SOD antibody of human,Rat

– use for western blotting, elisa, immunoprecipitation and immunohistochemistry

– Protein G affinity chromatography purification, purity :>95%

• Isotype: IgG
• mol wt:17 kDa

Application :

– Western blotting 1:100-500

– Immunohistochemistry 1:100-500

– ELISA 1:500-1000

– Optimal working dilutions must be determined by the end user.Anti-SOD抗体说明书，现货wb操作

Storage: Store at -20 ℃ for one year. Avoid repeated freeze/thaw cycles.

The lyophilized antibody is stable at room temperature for at least one month and for

greater than a year when kept at -20℃. When reconstituted in sterile pH 7.4 0.01M

PBS or diluent of antibody, the antibody is stable for at least six weeks at 2-4 ℃.

Important Note: This product as supplied is intended for research use only, not for

use in human, therapeutic or diagnostic applications.(用荧光色素标记的抗体）
?试管标号。
?试管内加入 20 μl 荧光色素标记的抗体。
?每个试管中加入 100 μl 准备好的细胞悬液（相当于 100 万细胞）。
?置于加盖的冰盒内，振荡并孵育 15－30 分钟。
?染色后每管中加入 1.5－2 ml 1x PBS 洗去多余抗体。
?用台式离心机 2000 RPM 离心 5 分钟。如果是细胞内染色，离心速度应增加到 3000 或 4000 RPM。
?小心吸去上清液，勿将沉淀冲起。
?1% 多聚甲醛 500 μl 重悬浮细胞。试管可以存放在避光处 24 小时（细胞内染色zui长时间）到 1 周（表面染色zui长时间）。
间接染色（用非荧光素标记的一抗和荧光色素标记的二抗）
?试管标号。
?试管内加入未标记的一抗。每管大约 1 μg。
?每个试管中加入 100 μl 准备好的细胞悬液（相当于 100 万细胞）。
?置于加盖的冰盒内，振荡并孵育 15－30 分钟。
?染色后每管中加入 1.5－2 ml 1x PBS 洗去多余抗体。
?用台离心机 2000 RPM 离心 5 分钟。如果是细胞内染色，离心速度应增加到 3000 或 4000 RPM。
?小心吸去上清液，勿将沉淀冲起。
?每管加入 100 μl 1x PBS。试管内加入荧光色素标记的二抗 0.5-1 μg。
?置于加盖的冰盒内，振荡并孵育 15－30 分钟。
?染色后每管中加入 1.5－2 ml 1x PBS 洗去多余抗体。