兔肿瘤坏死因子α(TNF-α)酶联免疫分析(ELISA)试剂盒
检测范围：8 ng/L -300 ng/L
试剂盒保存：2-8℃
有效期：6个月
实验目的：本试剂盒仅供研究使用,用于测定兔血清，血浆及相关液体样本中肿瘤坏死因子α（TNF-α）的含量。
实验原理：本试剂盒应用双抗体夹心法测定标本中兔肿瘤坏死因子α（TNF-α）水平。用纯化的兔肿瘤坏死因子α（TNF-α）抗体包被微孔板，制成固相抗体，往包被单抗的微孔中依次加入肿瘤坏死因子α，再与HRP标记的羊抗兔抗体结合，形成抗体-抗原-酶标抗体复合物，经过*洗涤后加底物TMB显色。TMB在HRP酶的催化下转化成蓝色，并在酸的作用下转化成zui终的黄色。颜色的深浅和样品中的兔肿瘤坏死因子α呈正相关。用酶标仪在450nm波长下测定吸光度（OD值），通过标准曲线计算样品中兔肿瘤坏死因子α（TNF-α）浓度。
试剂盒组成：
试剂盒组成 | 48孔配置 | 96孔配置 | 保存 |
说明书 | 1份 | 1份 |
|
封板膜 | 2片（48） | 2片（96） |
|
密封袋 | 1个 | 1个 |
|
酶标包被板 | 1×48 | 1×96 | 2-8℃保存 |
标准品：360ng/L | 0.5ml×1瓶 | 0.5ml×1瓶 | 2-8℃保存 |
标准品稀释液 | 1.5ml×1瓶 | 1.5ml×1瓶 | 2-8℃保存 |
酶标试剂 | 3 ml×1瓶 | 6 ml×1瓶 | 2-8℃保存 |
样品稀释液 | 3 ml×1瓶 | 6 ml×1瓶 | 2-8℃保存 |
显色剂A液 | 3 ml×1瓶 | 6 ml×1瓶 | 2-8℃保存 |
显色剂B液 | 3 ml×1瓶 | 6 ml×1瓶 | 2-8℃保存 |
终止液 | 3ml×1瓶 | 6ml×1瓶 | 2-8℃保存 |
浓缩洗涤液 | （20ml×20倍）×1瓶 | （20ml×30倍）×1瓶 | 2-8℃保存 |
Drug Names
Generic Name：Rabbit Tumor necrosis factor α(TNF-α) ELISA Kit.
Purpose
This kit allows for the determination of TNF-α concentrations in Rabbit serum, blood plasma, and other biological fluids.
Principle of the assay
The kit assay Rabbit TNF-α level in the sample，use Purified Rabbit TNF-α antibody to coat microtiter plate wells, make solid-phase antibody, then add TNF-α to wells, Combined antibody which With HRP labeled goat anti-rabbit become antibody - antigen - enzyme-antibody complex, after washing Compley, Add TMB substrate solution,TMB substrate becomes blue color At HRP enzyme-catalyzed, reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm. The concentration of TNF-α in the samples is then determined by comparing the O.D. of the samples to the standard curve.
Materials provided with the kit
Materials provided with the kit | 48determinations | 96 determinations | Storage |
User manual | 1 | 1 |
|
Closure plate membrane | 2 | 2 |
|
Sealed bags | 1 | 1 |
|
Microelisa stripplate | 1 | 1 | 2-8℃ |
Standard：360ng/L | 0.5ml×1 bottle | 0.5ml×1 bottle | 2-8℃ |
Standard diluent | 1.5ml×1 bottle | 1.5ml×1 bottle | 2-8℃ |
HRP-Conjugate reagent | 3ml×1 bottle | 6ml×1 bottle | 2-8℃ |
Sample diluent | 3ml×1 bottle | 6ml×1 bottle | 2-8℃ |
Chromogen Solution A | 3ml×1 bottle | 6ml×1 bottle | 2-8℃ |
Chromogen Solution B | 3ml×1 bottle | 6ml×1 bottle | 2-8℃ |
Stop Solution | 3ml×1 bottle | 6ml×1 bottle | 2-8℃ |
wash solution | （20ml×20 fold） ×1bottle | （20ml×30 fold） ×1bottle | 2-8℃ |
上海华壹生物科技有限公司
Shanghai HuaYi Bio-technology Co.,Ltd
