小鼠25羟基*3(25-OH-D3)酶联免疫分析（ELISA）试剂盒
Mouse 25-Dihydroxy vitamin D3 (25-OH-D3) ELISA Kit
试剂盒性能：
1.样品线性回归与预期浓度相关系数R值为0.92以上。
2.批内与批间应分别小于9%和15%
实验原理：本试剂盒用于测定小鼠血清，血浆及相关液体样本中25羟基*3(25-OH-D3)的含量。应用双抗体夹心法测定标本中小鼠25羟基*3(25-OH-D3)水平。用纯化的抗D3(25-OH-D3)抗体包被微孔板，制成固相抗体，往包被单抗的微孔中依次加入25羟基*3(25-OH-D3)，再与HRP标记的D3(25-OH-D3)抗体结合，形成抗体-抗原-酶标抗体复合物，经过*洗涤后加底物TMB显色。TMB在HRP酶的催化下转化成蓝色，并在酸的作用下转化成zui终的黄色。颜色的深浅和样品中的25羟基*3(25-OH-D3)呈正相关。用酶标仪在450nm波长下测定吸光度（OD值），通过标准曲线计算样品中小鼠25羟基*3(25-OH-D3)浓度。
Principle of the assay
The kit assay Mouse 25-OH-D3 level in the sample，use Purified Mouse 25-OH-D3 antibody to coat microtiter plate wells, make solid-phase antibody, then add 25-OH-D3 to wells, Combined 25-OH-D3 antibody which With HRP labeled , become antibody - antigen - enzyme-antibody complex, after washing Compley, Add TMB substrate solution,TMB substrate becomes blue color At HRP enzyme-catalyzed, reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm. The concentration of 25-OH-D3 in the samples is then determined by comparing the O.D. of the samples to the standard curve.
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上海华壹生物科技有限公司
Shanghai HuaYi Bio-technology Co.,Ltd
