菜豆凝集素（PHA）试剂盒实验注意事项：
- 试剂盒从冷藏环境中取出应在室温平衡15-30分钟后方可使用，酶标包被板开封后如未用完，板条应装入密封袋中保存。
- 浓洗涤液可能会有结晶析出，稀释时可在水浴中加温助溶，洗涤时不影响结果。
- 各步加样均应使用加样器，并经常校对其准确性，以避免试验误差。一次加样时间控制在5分钟内，如标本数量多，推荐使用排枪加样。
- 请每次测定的同时做标准曲线，做复孔。如标本中待测物质含量过高（样本OD值大于标准品孔*孔的OD值），请先用样品稀释液稀释一定倍数（n倍）后再测定，计算时请zui后乘以总稀释倍数（×n×5）。
- 封板膜只限一次性使用，以避免交叉污染。
- 底物请避光保存。
- 严格按照说明书的操作进行，试验结果判定必须以酶标仪读数为准.
- 所有样品，洗涤液和各种废弃物都应按传染物处理。
- 本试剂不同批号组分不得混用,如与英文说明书有异，以英文说明书为准。
Phaseolus vulgaris agglutinin（ PHA） ELISA Kit.
Important notes
- The kit takes out from the refrigeration environment should be balanced 15-30 minutes in the room temperature, ELISA plates coated if has not use up after opened, the plate should be stored in Sealed bag.
- washing buffer will Crystallization separation, it can be heated the water helps dissolve when dilute . Washing does not affect the result.
- add Sample with sampler Each step, And proofread its accuracy frequently, avoids the experimental error. add sample within 5 mins, if the number of sample is much , recommend to use Volley .
- if the testing material content is excessively higher (The sample OD is bigger than the first standard well ),please dilute Sample (n-fold), Please diluente and multiplied by the dilution factor.（×n×5）.
- Closure plate membrane only limits the disposable use, to avoid cross-contamination.
- The substrate evade the light preservation.
- Please according to use instruction strictly, The test result determination must take the microtiter plate reader as a standard.
- All samples, washing buffer and each kind of reject should according to infective material process.
- Do not mix reagents with those from other lots.
