大鼠血栓素B2(TXB2)试剂盒
实验原理：
本试剂盒用于测定大鼠血清，血浆及相关液体样本中血栓素B2(TXB2)的含量。应用双抗体夹心法测定标本中大鼠血栓素B2(TXB2)水平。用纯化的大鼠血栓素B2(TXB2)抗体包被微孔板，制成固相抗体，往包被单抗的微孔中依次加入血栓素B2(TXB2)，再与HRP标记的血栓素B2(TXB2)抗体结合，形成抗体-抗原-酶标抗体复合物，经过*洗涤后加底物TMB显色。TMB在HRP酶的催化下转化成蓝色，并在酸的作用下转化成zui终的黄色。颜色的深浅和样品中的血栓素B2(TXB2)呈正相关。用酶标仪在450nm波长下测定吸光度（OD值），通过标准曲线计算样品中大鼠血栓素B2(TXB2)浓度。
Rat Thromboxane B2 (TXB2) ELISA Kit.
Principle of the assay
The kit assay Rat TXB2 level in the sample，use Purified Rat TXB2 antibody to coat microtiter plate wells, make solid-phase antibody, then add TXB2 to wells, Combined TXB2 antibody which With HRP labeled, become antibody - antigen - enzyme-antibody complex, after washing Compley, Add TMB substrate solution,TMB substrate becomes blue color At HRP enzyme-catalyzed, reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm. The concentration of TXB2 in the samples is then determined by comparing the O.D. of the samples to the standard curve.
上海华壹生物科技有限公司
Shanghai HuaYi Bio-technology Co.,Ltd
