小鼠促卵泡激素（FSH）酶联免疫分析（ELISA）试剂盒
Mouse Follicle-stimulating hormone (FSH) ELISA Kit
实验原理：本试剂盒仅供研究使用，用于测定小鼠血清，血浆及相关液体样本中促卵泡激素（FSH）的含量。应用双抗体夹心法测定标本中小鼠促卵泡激素（FSH）水平。用纯化的小鼠促卵泡激素（FSH）抗体包被微孔板，制成固相抗体，往包被单抗的微孔中依次加入促卵泡激素（FSH），再与HRP标记的FSH抗体结合，形成抗体-抗原-酶标抗体复合物，经过*洗涤后加底物TMB显色。TMB在HRP酶的催化下转化成蓝色，并在酸的作用下转化成zui终的黄色。颜色的深浅和样品中的小鼠促卵泡激素（FSH）呈正相关。用酶标仪在450nm波长下测定吸光度（OD值），通过标准曲线计算样品中小鼠促卵泡激素（FSH）浓度。
Principle of the assay
The kit assay Mouse FSH level in the sample，use Purified Mouse FSH antibody to coat microtiter plate wells, make solid-phase antibody, then add FSH to wells, Combined FSH which With HRP labeled , become antibody - antigen - enzyme-antibody complex, after washing Compley, Add TMB substrate solution,TMB substrate becomes blue color At HRP enzyme-catalyzed, reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm. The concentration of FSH in the samples is then determined by comparing the O.D. of the samples to the standard curve.
ELISA试剂盒供应商：上海华壹生物科技有限公司
Shanghai HuaYi Bio-technology Co.,Ltd
