植物钙调素（CaM）酶联免疫分析（ELISA）试剂盒
实验原理：本试剂盒本试剂盒用于测定植物组织，细胞及其它相关样本中植物钙调素（CaM）含量。应用双抗体夹心法测定标本中植物钙调素（CaM）水平。用纯化的植物钙调素（CaM）抗体包被微孔板，制成固相抗体，往包被单抗的微孔中依次加入植物钙调素（CaM）抗原，再与HRP标记的植物钙调素（CaM）抗体结合，形成抗体-抗原-酶标抗体复合物，经过*洗涤后加底物TMB显色。TMB在HRP酶的催化下转化成蓝色，并在酸的作用下转化成zui终的黄色。颜色的深浅和样品中的植物钙调素（CaM）呈正相关。用酶标仪在450nm波长下测定吸光度（OD值），通过标准曲线计算样品中植物钙调素（CaM）抗原浓度。
Drug Names
Generic Name：Plant calmodulin (CaM) ELISA Kit.
Purpose
This kit allows for the determination of CaM concentrations in Plant tissue ,cell and other samples.
Principle of the assay
The kit assay Plant CaM level in the sample，use Purified Plant CaM antibody to coat microtiter plate wells, make solid-phase antibody, then add CaM to wells, Combined CaM antibody which With HRP labeled , become antibody - antigen - enzyme-antibody complex, after washing Compley, Add TMB substrate solution,TMB substrate becomes blue color At HRP enzyme-catalyzed, reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm. The concentration of CaM in the samples is then determined by comparing the O.D. of the samples to the standard curve.
上海华壹生物科技有限公司
Shanghai HuaYi Bio-technology Co.,Ltd
