小鼠双氢睾酮(DHT)ELISA试剂盒
实验原理：
本试剂盒用于测定小鼠血清，血浆及相关液体样本中双氢睾酮(DHT)的含量。应用双抗体夹心法测定标本中小鼠双氢睾酮(DHT)水平。用纯化的小鼠双氢睾酮(DHT)抗体包被微孔板，制成固相抗体，往包被单抗的微孔中依次加入双氢睾酮(DHT)，再与HRP标记的双氢睾酮(DHT)抗体结合，形成抗体-抗原-酶标抗体复合物，经过*洗涤后加底物TMB显色。TMB在HRP酶的催化下转化成蓝色，并在酸的作用下转化成zui终的黄色。颜色的深浅和样品中的双氢睾酮(DHT)呈正相关。用酶标仪在450nm波长下测定吸光度（OD值），通过标准曲线计算样品中小鼠双氢睾酮(DHT)浓度。
Mouse Dihydrotestosterone(DHT)ELISA Kit
Purpose:
This kit allows for the determination of DHT concentrations in Mouse serum, blood plasma, and other biological fluids.
Principle of the assay
The kit assay Mouse DHT level in the sample，use Purified Mouse DHT to coat microtiter plate wells, make solid-phase antibody, then add DHT to wells, Combined DHT antibody which With HRP labeled , become antibody - antigen - enzyme-antibody complex, after washing Compley, Add TMB substrate solution,TMB substrate becomes blue color At HRP enzyme-catalyzed, reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm. The concentration of DHT in the samples is then determined by comparing the O.D. of the samples to the standard curve.
试剂盒供应商：上海华壹生物科技有限公司
