牛磷酸果糖激酶(PFK)ELISA试剂盒实验原理：本试剂盒用于测定牛血清，细胞上清及相关液体样本中磷酸果糖激酶(PFK)的活性。应用双抗体夹心法测定标本中牛磷酸果糖激酶(PFK)水平。用纯化的牛磷酸果糖激酶(PFK)抗体包被微孔板，制成固相抗体，往包被单抗的微孔中依次加入磷酸果糖激酶(PFK)，再与HRP标记的磷酸果糖激酶(PFK)抗体结合，形成抗体-抗原-酶标抗体复合物，经过*洗涤后加底物TMB显色。TMB在HRP酶的催化下转化成蓝色，并在酸的作用下转化成zui终的黄色。颜色的深浅和样品中的磷酸果糖激酶(PFK)呈正相关。用酶标仪在450nm波长下测定吸光度（OD值），通过标准曲线计算样品中牛磷酸果糖激酶(PFK)浓度。
Bovine Phosphofructokinase (PFK) ELISA Kit
Principle of the assay:
This kit allows for the determination of PFK concentrations in Bovine serum, cell culture supernatant, and other biological fluids.Assay Bovine PFK level in the sample，use Purified Bovine PFK antibody to coat microtiter plate wells, make solid-phase antibody, then add PFK to wells, Combined PFK antibody which With HRP labeled , become antibody - antigen - enzyme-antibody complex, after washing Compley, Add TMB substrate solution,TMB substrate becomes blue color At HRP enzyme-catalyzed, reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm. The concentration of PFK in the samples is then determined by comparing the O.D. of the samples to the standard curve.
试剂盒供应商：上海华壹生物科技有限公司
