Mouse Testosterone(T)ELISA KIT
小鼠睾酮(T)酶联免疫分析(elisa)试剂盒实验原理 ：
本试剂盒用于测定小鼠血清，血浆及相关液体样本中睾酮（T）的含量。应用双抗体夹心法测定标本中小鼠睾酮（T）水平。用纯化的小鼠睾酮（T）抗
体包被微孔板，制成固相抗体，往包被单抗的微孔中依次加入睾酮（T），再与 HRP 标记的睾酮（T）抗体结合，形成抗体-抗原-酶标抗体复合物，经过*洗涤后加底物 TMB 显色。TMB 在 HRP 酶的催化下转化成蓝色，并在酸的作用下转化成zui终的黄色。颜色的深浅和样品中的睾酮（T）呈正相关。用酶标仪在 450nm 波长下测定吸光度（OD 值），通过标准曲线计算样品中小鼠睾酮（T）浓度。
Principle of the assay:
T he kit assay Mouse T level in the sample，use Purified Mouse T to coat microtiter plate
wells, make solid-phase antibody, then add T to wells, Combined T antibody which With HRP
labeled , become antibody - antigen - enzyme-antibody complex, after washing Compley,
Add TMB substrate solution,TMB substrate becomes blue color At HRP enzyme-catalyzed,
reaction is terminated by the addition of a sulphuric acid solution and the color change is
measured spectrophotometrically at a wavelength of 450 nm. The concentration of T in the
samples is then determined by comparing the O.D. of the samples to the standard curve.
小鼠试剂盒供应商：上海华壹生物科技有限公司
