大鼠胃泌素(Gastrin)ELISA试剂盒
Rat Gastrin ELISA Kit
实验目的：本试剂盒仅供研究使用，用于测定大鼠血清，血浆及相关液体样本中胃泌素(Gastrin)的含量。
实验原理：本试剂盒应用双抗体夹心法测定标本中大鼠胃泌素(Gastrin)水平。用纯化的大鼠胃泌素(Gastrin)抗体包被微孔板，制成固相抗体，往包被单抗的微孔中依次加入胃泌素(Gastrin)，再与HRP标记的胃泌素(Gastrin)抗体结合，形成抗体-抗原-酶标抗体复合物，经过*洗涤后加底物TMB显色。TMB在HRP酶的催化下转化成蓝色，并在酸的作用下转化成zui终的黄色。颜色的深浅和样品中的胃泌素(Gastrin)呈正相关。用酶标仪在450nm波长下测定吸光度（OD值），通过标准曲线计算样品中大鼠胃泌素(Gastrin)浓度。
Purpose
This kit allows for the determination of Gastrin concentrations in Rat serum, blood plasma, and other biological fluids.
Principle of the assay
The kit assay Rat Gastrin level in the sample，use Purified Rat Gastrin antibody to coat microtiter plate wells, make solid-phase antibody, then add Gastrin to wells, Combined Gastrin which With HRP labeled , become antibody - antigen - enzyme-antibody complex, after washing Compley, Add TMB substrate solution,TMB substrate becomes blue color At HRP enzyme-catalyzed, reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm. The concentration of Gastrin in the samples is then determined by comparing the O.D. of the samples to the standard curve.
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