大鼠胰岛素(INS)含量测定ELISA试剂盒
Generic Name：Rat Insulin(INS) ELISA Kit
检测范围：
0.3 mU/L -15 mU/L
试剂盒性能：
1.样品线性回归与预期浓度相关系数R值为0.92以上。
2.批内与批间应分别小于9%和15%
实验原理：本试剂盒应用双抗体夹心法测定标本中大鼠胰岛素(INS)水平。用纯化的大鼠胰岛素(INS)抗体包被微孔板，制成固相抗体，往包被单抗的微孔中依次加入(INS),再与HRP标记的胰岛素（INS）抗体结合，形成抗体-抗原-酶标抗体复合物，经过*洗涤后加底物TMB显色。TMB在HRP酶的催化下转化成蓝色，并在酸的作用下转化成zui终的黄色。颜色的深浅和样品中的胰岛素(INS)呈正相关。用酶标仪在450nm波长下测定吸光度（OD值），通过标准曲线计算样品中大鼠(INS)浓度。
Principle of the assay
The kit assay Rat INS level in the sample，use Purified Rat INS to coat microtiter plate wells, make solid-phase antibody, then add INS to wells, Combined INS which With HRP labeled , become antibody - antigen - enzyme-antibody complex, after washing Compley, Add TMB substrate solution,TMB substrate becomes blue color At HRP enzyme-catalyzed, reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm. The concentration of INS in the samples is then determined by comparing the O.D. of the samples to the standard curve.
ELISA试剂盒上海供应商:华壹生物
