猴睾酮ELISA试剂盒
实验原理：本试剂盒用于测定猴血清，粪便及相关液体样本中睾酮（T）的含量。应用双抗体夹心法测定标本中猴睾酮(T)水平。用纯化的猴睾酮(T)抗体包被微孔板，制成固相抗体，往包被单抗的微孔中依次加入睾酮(T)，再与HRP标记的睾酮(T)抗体结合，形成抗体-抗原-酶标抗体复合物，经过*洗涤后加底物TMB显色。TMB在HRP酶的催化下转化成蓝色，并在酸的作用下转化成zui终的黄色。颜色的深浅和样品中的睾酮(T)呈正相关。用酶标仪在450nm波长下测定吸光度（OD值），通过标准曲线计算样品中猴睾酮(T)浓度。
Monkey Testosterone(T) ELISA Kit
Principle of the assay:
The kit assay Monkey T level in the sample，use Purified Monkey T to coat microtiter plate wells, make solid-phase antibody, then add T to wells, Combined T antibody which With HRP labeled , become antibody - antigen - enzyme-antibody complex, after washing Compley, Add TMB substrate solution,TMB substrate becomes blue color At HRP enzyme-catalyzed, reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm. The concentration of T in the samples is then determined by comparing the O.D. of the samples to the standard curve.
酶联免疫试剂盒供应商：上海华壹生物科技有限公司
