HE035
小鼠氧化型*(GSSG)ELISA试剂盒保存：2-8℃
有效期：6 个月
Mouse oxidizided glutathione,GSSG ELISA Kit
实验计算：
以标准物的浓度为横坐标，OD值为纵坐标，在坐标纸上绘出标准曲线，根据样品的OD值由标
准曲线查出相应的浓度；再乘以稀释倍数；或用标准物的浓度与OD值计算出标准曲线的直线
回归方程式，将样品的OD值代入方程式，计算出样品浓度，再乘以稀释倍数，即为样品的实
际浓度。
Calculate:
Take the standard density as the horizontal, the OD value for the vertical ,draw
the standard curve on graph paper, Find out the corresponding density according
to the sample OD value by the Sample curve, multiplied by the dilution multiple,
or calculate the straight line regression equation of the standard curve with the
standard density and the OD value ,with the sample OD value in the equation,
calculate the sample density, multiplied by the dilution factor, the result is
the sample actual density.
小鼠氧化型*(GSSG)ELISA试剂盒保存
实验注意事项：
1． 试剂盒从冷藏环境中取出应在室温平衡 15-30 分钟后方可使用，酶标包被板开封后如未
用完，板条应装入密封袋中保存。
2． 浓洗涤液可能会有结晶析出，稀释时可在水浴中加温助溶，洗涤时不影响结果。
3． 各步加样均应使用加样器，并经常校对其准确性，以避免试验误差。一次加样时间
控制在 5 分钟内，如标本数量多，*使用排枪加样。
4． 请每次测定的同时做标准曲线，做复孔。如标本中待测物质含量过高（样本 OD 值
大于标准品孔*孔的 OD 值），请先用样品稀释液稀释一定倍数（n 倍）后再测定，计
算时请zui后乘以总稀释倍数（×n×5）。
5． 封板膜只限一次性使用，以避免交叉污染。
6． 底物请避光保存。
7． 严格按照说明书的操作进行，试验结果判定必须以酶标仪读数为准.
8． 所有样品，洗涤液和各种废弃物都应按传染物处理。
9． 本试剂不同批号组分不得混用。
Important notes:
1. The kit takes out from the refrigeration environment should be balanced 15-30 minutes in the room temperature, ELISA plates coated if has not use up after opened, the plate should be stored in Sealed bag.
2. washing buffer will Crystallization separation, it can be heated the water helps dissolve when dilute . Washing does not affect the result.
3. add Sample with sampler Each step, And proofread its accuracy frequently,avoids the experimental error. add sample within 5 mins, if the number of sample is much,recommend to use Volley .
4. if the testing material content is excessively higher (The sample OD is bigger than the first standard well ),please dilute Sample (n-fold), Please diluente and multiplied by the dilution factor.（×n×5）.
5. Closure plate membrane only limits the disposable use, to avoid cross-contamination.
6. The substrate evade the light preservation.
7. Please according to use instruction strictly, The test result determination must take the microtiter plate reader as a standard.
8. All samples, washing buffer and each kind of reject should according to infective material process.
9. Do not mix reagents with those from other lots.
小鼠氧化型*(GSSG)ELISA试剂盒保存
试剂盒供应商：上海华壹生物科技有限公司
