Rabbit  Anti-Troponin I

Catalog Number :  HZ72819        Quantity size :   0.1ml  （dilute with pH 7.4    0.01 M PBS or antibody diluent ）

Background: Actin is a highly conserved protein that is expressed in all eukaryotic cells. Actin filaments can form both stable and labile structures and are crucial  components of microvilli and the contractile apparatus of muscle cells. Myosin is a hexamer of two heavy chains （MHC） and four light chains （MLC）  that interacts with Actin to generate the force for diverse cellular movements, including cytokinesis, phagocytosis and muscle contraction. Troponin facilitates the interaction between Actin and Myosin by binding to calcium. Troponin is made up of at least two subunits, which are divergent in cardiac muscle, fast skeletal muscle and slow skeletal muscle. Structures of skeletal muscle troponin are composed of Troponin C （the sensor）, Troponin I （the regulator） and Troponin T （the link to the muscle thin filament）. Troponin C is dumbbell-shaped and has a hydrophobic pocket that increases the contractile force of muscle fibers. Troponin C has two isoforms: fast and slow. Fast

Troponin C has two calcium binding sites, while slow/cardiac Troponin C has a single calcium binding site.

pecificity :   

–        Anti- Troponin I  is a rabbit polyclonal antibody unconjugated                

–         specific for  Troponin I  of human, mouse, rat          

–         use for western blotting,elisa,immunoprecipitation and immunohistochemistry

–         Protein G affinity chromatography purification, purity :>95%

–         Isotype:  IgG

–         mol wt:  29kDa

Application :  

–        Western blotting 1:100-500                

–         Immunohistochemistry 1:100-500                 

–         ELISA 1:500-1000                  

–         Optimal working dilutions must  be determined by the end user.

Storage:  Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. The  lyophilized antibody is stable at room temperature for at least one month and for greater  than a year when kept at -20℃. When reconstituted in sterile pH 7.4 0.01M PBS or diluent  of antibody, the antibody is stable for at least six weeks at 2-4 ℃

 Important Note: This product as supplied is intended for research use only, not for use in  human, therapeutic or diagnostic applications.

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