小鼠突触素(SYP)ELISA检测试剂盒免费代测子科生物现货供应,凡购买子科生物子科生物任何一款ELISA酶联免疫分析检测试剂盒,都可以享受免费代测服务,外地客户可以由技术老师的指导下正确放置好样本的保存方式后快递邮寄到我公司技术部,本地客户可以享受免费上门取样服务!
?产品名称:小鼠突触素(SYP)ELISA检测试剂盒,小鼠突触素(SYP)酶联免疫试剂盒,小鼠突触素(SYP)ELISA检测试剂盒说明书,深圳子科暑期7折*
?英文名称:mouse Synaptophysin (SYP) ELISA Kit
?产品规格:96T(人份)/48T(人份)(单孔检测分别可以检测90份样本,42分样本,一般都需要留6个孔做标准曲线)。
?检测方法:酶联免疫分析ELISA方法
?有效期: 4 个月(4℃);8 个月(-20℃)。
?保存方法: 2-8℃(频繁使用时); -20℃(长时间不用时)。
?品牌:子科生物ZIKER,美国R&D,美国immonoway,美国sciencell,德国IBL
凡购买我们公司的ELISA相关系列产品,都可以享受免费代测服务,只收取试剂盒的相关费用,实验检测和实验涉及到的试剂全部都是免费提供,并可以提供zui终实验数据分析工作。需要代测服务的客户,您只需要准备收集好您的样本,邮寄到我公司实验室,本市的销售人员可以上门取样本,我公司收到样本之后正常是在一周内给您检测报告结果。
小鼠突触素(SYP)ELISA检测试剂盒标本的处理
可用作 ELISA 测定的标本十分广泛,体液(如血清)、分泌物(唾液)和排泄物(如尿液)、 细胞培养上清、细胞、组织等均可作标本以测定其中某种成份。有些标本可直接进行测定,有些 则需经预处理。
● 血清:
室温血液自然凝固 10-20 分钟后,离心 20 分钟左右( 2000-3000 转 / 分)。收集上清。如 有沉淀形成,应再次离心。
● 血浆:
应根据试剂盒的要求选择 EDTA 、柠檬酸钠或肝素作为抗凝剂,加入 10 %( v/v )抗凝剂 ( 0.1M 柠檬酸钠或 1% heparin 或 2.0%EDTA.Na2) 混合 10-20 分钟后,离心 20 分钟左右 ( 2000-3000 转 / 分)。仔细收集上清。如有沉淀形 成,应再次离心。
●尿液、胸腹水、脑脊液:
用无菌管收集。离心 20 分钟左右( 2000-3000 转 / 分)。仔细收集上清。如有沉淀形成, 应再次离心。
●细胞培养上清:
检测分泌性的成份时,用无菌管收集。离心 20 分钟左右( 2000-3000 转 / 分)。仔细收集 上清。检测细胞内的 成份时,用 PBS ( PH7.2-7.4 )稀释细胞悬液,细胞浓度达到 100 万 /ml 左右。通过反复冻融,以使细胞破坏并放出细胞内成份。离心 20 分钟左右( 2000-3000 转 / 分)。仔细收集上清。保存过程中如有沉淀形成,应再次离心。
●细胞:
检测细胞的成份时,对于贴壁细胞,去除培养液,用PBS、理盐水或无血清培养液洗一遍。加 入适量裂解液。用枪吹打数下,使裂解液和细胞充分接触。通常裂解液接触细胞10 秒后,细胞 就会被裂解。对于悬浮细胞,离心收集细胞,用PBS、理盐水或无血清培养液洗一遍.加入适 量裂解液, 用枪吹打把细胞吹散。用手指轻弹以充分裂解细胞。充分裂解后应没有明显的细胞沉 淀。如果细胞量较多,必需分装然后再裂解。充分裂解后,10000-14000g离心3-5 分钟,取上 清,即可进行后续操作。
●组织标本:
切割标本后,称取重量。加入一定量的 PBS 或组织蛋白萃取试剂,缓冲液中可加入蛋白酶抑制 剂。用手工或匀浆器将标本匀浆充分。离心 20 分钟左右( 2000-3000 转 / 分)。仔细收集 上清置于 -20 度或 - 70 度保存,如有必要,可以将样品浓缩干燥。分装后一份待检测,其余冷冻备用。
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