产品名称：Anti-C12orf72抗体,12号染色体开放阅读框72抗体

产品编号：BYK-18117R
英文名称：Anti-C12orf72
中文名称：12号染色体开放阅读框72抗体
英文别名：hypothetical protein LOC254013;

C12orf72;

Chromosome 12 open reading frame 72;

MGC50559;

UPF0516 protein C12orf72.
英文介绍：GTPase-activating proteins (GAPs) accelerate the intrinsic rate of GTP hydrolysis of Ras-related proteins, resulting in downregulation of their active form. ARHGAP36 (Rho GTPase activating protein 36) is a 547 amino acid protein that contains one Rho-GAP domain. Conserved in chimpanzee, dog, cow, mouse and rat, ARHGAP36 exists as five alternatively spliced isoforms and is encoded by a gene that maps to human chromosome Xq26.1. Chromosome X consists of nearly 153 million base pairs encoding approximay 1,000 genes. More than one copy of the X chromosome with a Y chromosome causes Klinefelter's syndrome. A single copy of X alone leads to Turner's syndrome. More than 2 copies of the X chromosome, in the absence of a Y chromosome, is known as Triple X syndrome. Color blindness, hemophilia, and Duchenne muscular dystrophy are X chromosome-linked conditions that affect males more frequently because males carry a single X chromosome.

产品规格：0.1ml/0.2ml

注意事项:This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.

此抗体本公司有单抗和多抗，可用于免疫组化（IHC），免疫印迹（WB），酶联免疫(ELISA)，IF、IP、流式细胞术等实验（可来电索取说明）
此产品本公司有标记的一抗出售，相关标记有：Alexa Fluor 350 标记、Alexa Fluor 488 标记、Alexa Fluor 555 标记、Alexa Fluor 647 标记、AP标记、APC标记、Biotin标记、Cy3标记、Cy5标记、Cy5.5标记、Cy7标记、FITC标记、Gold标记、HRP标记、PE标记、PE-Cy3标记、PE-CY5标记、PE-CY5.5标记、PE-CY7标记、RBITC标记

保存条件：Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C.

Anti-C12orf72抗体,12号染色体开放阅读框72抗体免疫组化操作流程
1 脱蜡和水化：脱蜡前应将组织芯片在室温中放置60分钟或60℃恒温箱中烘烤20分钟。 a 组织芯片置于二甲苯中浸泡10分钟,更换二甲苯后在浸泡10分钟
b 无水乙醇中浸泡五分钟
c 95%乙醇中浸泡五分钟
d 75%乙醇中浸泡五分钟
2 抗原修复：用于福尔马林固定的石蜡包埋组织芯片：
A 抗原热修复
a 高压热修复 在沸水中加入EDTA（ph8.0）或0.01m*缓冲溶液（ph6.0）.盖上不锈钢锅盖，但不能锁定。将玻片置于金属染色加上，缓慢加压，是玻片在缓冲液中浸泡五分钟，然后将盖子锁定，小阀门将会升起来。10分钟后除去热源，置入凉水中，当小阀门沉下去后打开盖子。此方法适用于较难检测或核抗原的抗原修复。
b 沸热修复 电炉或水浴锅加热0.01*缓冲液（ph6.0）至95℃左右，放入组织芯片加热10-15分钟。
C 微波炉加热 在微波炉里加热0.01*缓冲液（ph6.0）至沸腾后将组织芯片放入，断电，间隔5-10分钟，反复1-2次。适用的抗原：
Bcl-2.Bax.AR.PR.C-fos.x-jum.z-kit.c-myc.E-cadherin.
ChromograninA.Cyclin.ER.Heatshock.Protein.HPV.Ki-67.MDMZ.P53.P34.P15.P-glycoprotein.PKC.PCNA.ras.Rb.等
B 酶消化方法 
常用0.1%*和0.4%*液。*使用前预热37℃，消化时间约为5-30分钟。适用于被固定遮蔽的抗原：包括 Collagen.GFAP.
Complement.Cytokeratin.C-erB-2.LCA.LN.等

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