产品名称：Anti-CNG3抗体,视锥感光细胞环磷酸鸟苷门控通道蛋白CNG3抗体技术说明

产品编号：BYK-10772R
英文名称：Anti-CNG3
中文名称：视锥感光细胞环磷酸鸟苷门控通道蛋白CNG3抗体
英文别名：ACHM2; CCNC1; CCNCa; CCNCalpha; CNCG3; CNG 3; CNG-3; CNG channel alpha 3; CNG channel alpha-3; CNG-3; CNG3; CNGA3; Cnga3 cyclic nucleotide gated channel alpha 3; CNGA3_HUMAN; Cone photoreceptor cGMP gated channel; Cone photoreceptor cGMP gated channel alpha subunit; Cone photoreceptor cGMP gated channel subunit alpha; Cone photoreceptor cGMP-gated channel subunit alpha; Cyclic nucleotide gated cation channel alpha 3; Cyclic nucleotide gated channel alpha 3; Cyclic nucleotide gated channel olfactory 3; Cyclic nucleotide gated channel rod photoreceptor subunit alpha; Cyclic nucleotide-gated cation channel alpha-3; Cyclic nucleotide-gated channel alpha-3.
英文介绍：Cyclic nucleotide-gated (CNG) cation channels are heteromeric complexes made up of principal alpha and modulatory beta subunits. The alpha subunits consist of CNG1-3 and form functional cation channels by themselves. The beta subunits consist of CNG4-6 and, unlike the alpha subunits, do not form functional channels, but rather modify the properties of channels. CNG channels are essential components of olfactory and visual transduction. In olfactory neurons, CNG2, CNG4.3 and CNG5 form Ca2+ permeable channels, which open and depolarize the cell in response to cAMP. In rod photoreceptors, CNG1 and CNG4.1 combine to form Ca ion permeable channels, which give rise to a current in response to cGMP. CNG3 and CNG6 are expressed in cone receptors and may combine to form a native cGMP-activated channel. CNG channels have been implicated in other areas. CNG1 is also expressed in medium-sized and small-sized arteries, suggesting a role for CNG in the regulation of arterial blood pressure and of blood supply to different regions. CNG1, CNG4.1 and CNG4.2 have been detected in the rat pineal gland. CNG2, CNG4.3 and CNG5 are present in GT1 cell lines and may play a role in the secretion of gonadotropin-releasing hormone.

产品规格：0.1ml/0.2ml

注意事项:This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.

此抗体本公司有单抗和多抗，可用于免疫组化（IHC），免疫印迹（WB），酶联免疫(ELISA)，IF、IP、流式细胞术等实验（可来电索取说明）
此产品本公司有标记的一抗出售，相关标记有：Alexa Fluor 350 标记、Alexa Fluor 488 标记、Alexa Fluor 555 标记、Alexa Fluor 647 标记、AP标记、APC标记、Biotin标记、Cy3标记、Cy5标记、Cy5.5标记、Cy7标记、FITC标记、Gold标记、HRP标记、PE标记、PE-Cy3标记、PE-CY5标记、PE-CY5.5标记、PE-CY7标记、RBITC标记

保存条件：Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C.

Anti-CNG3抗体,视锥感光细胞环磷酸鸟苷门控通道蛋白CNG3抗体技术说明免疫组化染色：
SP法
（1） 脱蜡、水化 （2）PBS洗2-3次各5分钟 （3）3%H2O2（80%甲醇）滴加在
TMA上，室温静置10分钟 （4）PBS洗2-3次各5分钟 （5）抗原修复
（6）PBS洗2-3次各5分钟 （7）滴加正常山羊血清封闭液，室温20分钟，甩去多余液体（8）滴加Ι抗50μl,室温静置1小时或4℃过夜或37℃1小时（9）4℃过夜后需在37℃复温45分钟（10）PBS洗3次每次2分钟
（11）滴加Ⅱ抗45-50μl,室温静置或37℃1小时（12）Ⅱ抗中可加入0.05%的
tween-20.（13）PBS洗3次各5分钟（14）DAB显色5-10分钟，在显微镜下掌握染色程度 （15）PBS或自来水冲洗10分钟 （16）苏木精复染2分钟，盐酸酒精分化（17）自来水冲洗10-15分钟 （18）脱水、透明、封片、镜检。
SABC法
（1） 脱蜡、水化 （2）PBS洗2次各5分钟 （3）用蒸馏水或PBS配制新鲜的3%H2O2，室温封闭5-10分钟，用蒸馏水洗3次（4）抗原修复
（5） PBS洗5分钟 （6）滴加正常山羊血清封闭液，室温20分钟，甩去多余液体（7）滴加Ι抗50μl,室温静置1小时或4℃过夜或37℃1小时
（8）PBS洗3次各2分钟（9）滴加*化Ⅱ抗，20℃-37℃ 20分钟
（10）PBS洗3次各2分钟 （11）滴加试剂SABC 20℃-30℃ 20分钟
（12） PBS洗4次各5分钟 （13） DAB显色：试剂盒或自配显色剂显色
（14）脱水、透明、封片、镜检。

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