哈希MEL850大肠杆菌,总大肠菌群,总细菌,酵母肠球菌测定仪 大肠杆菌检测仪
MEL 面议污水处理设备 污泥处理设备 水处理过滤器 软化水设备/除盐设备 纯净水设备 消毒设备|加药设备 供水/储水/集水/排水/辅助 水处理膜 过滤器滤芯 水处理滤料 水处理剂 水处理填料
赛默飞世尔科技(中国)有限公司集团
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Thermo Scientific Remel MUG 纸片用于采用荧光测定法对大肠杆菌进行推断性鉴定。
Godsey et al. supported their findings in an evaluation of methods based on microbial enzyme activity profiles3. Most strains of E. coli produce an enzyme β-D-glucuronidase. In the indole test, the enzyme tryptophanase attacks tryptophan molecule on its side chain, leaving the aromatic ring in the form of indole. The indole is then detected by addition of ρ-dimethylamino-benzaldehyde (Kavocs’ Reagent) which produces a red color. β-D-glucuronidase is an enzyme that cleaves the substrate 4-methylumbelliferyl-β-D-glucuronide (MUG) and thereby produces a fluorescent product called methylumbelliferone4. This compound is detectable using a long wave ultraviolet light (360 nm).
Interpretation:
MUG Disk Test (Direct or Tube Test):
Positive test - Blue fluorescence observed under 360 nm UV light
Negative test - No fluorescence observed under 360 nm UV light
Indole Test:
Positive test - Red color development on the disk or in the tube
Negative test - No red color development on the disk or in the tube
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