几种难溶性磷脂的溶解方法及溶解度数据
大部分常用的合成磷脂都溶液HCCl3等有机溶剂,但有一些常用合成磷脂的既不溶于水,也不溶于HCCl3和其他有机溶剂,就连DMSO也无法溶解,因此有一些客户买到这种难溶的合成磷脂,常常去摸索溶解方法及溶解度造成了时间和材料的浪费,西安凯新生物在这里列出一些常用的难溶性磷脂的溶解方法和溶解度数据。
DPPE
CAS: 923-61-5
Molecular Weight:692
Transition Temp: 63°C (Tm), 118°C (Th)
Dissolving method: CHCl3/MeOH/H2O (65:35:8)
Solubility: 25mg/ml
DMPE
CAS: 998-07-2
Molecular Weight:635
Transition Temp: 50°C
Dissolving method: CHCl3/MeOH/H2O (65:35:8)
Solubility: 25mg/ml
DSPE
CAS: 1069-79-0
Molecular Weight:748
Transition Temp: 74°C (Tm), 100°C (Th)
Dissolving method: CHCl3/MeOH/H2O (65:35:8)
Solubility: 25mg/ml
DPPG
CAS: 200880-42-8
Molecular Weight:801
Transition Temp: 55°C
Dissolving method: CHCl3/MeOH/H2O (65:35:8)
Solubility: 25mg/ml
DSPG
CAS: 200880-41-7
Molecular Weight:744
Transition Temp: 41°C
Dissolving method: CHCl3/MeOH/H2O (65:35:8)
Solubility: 25mg/ml
DPPS
CAS: 145849-32-7
Molecular Weight:757
Transition Temp: 54°C
Dissolving method: CHCl3/MeOH/H2O (65:35:8)
Solubility: 20mg/ml
DSPE-PEG(2000)-Folate
Molecular Weight:3200
Dissolving method: Chloroform/Methanol (9:1)
Solubility: 10mg/ml
其他磷脂的溶解度数据:
DSPA and DPPA is soluble in chloroform 1.6mg/ml
DPPS is soluble in chloroform 0.5mg/ml
DMPE is soluble in chloroform 3mg/ml
POPE is soluble in chloroform 25mg/ml
POPG is soluble in chloroform 25mg/ml
DSPE-PEG2000 is soluble in chloroform 50mg/ml in hot water 10ng/ml
DSPE-PEG2000-COOH is soluble in chloroform 100mg/ml in hot water 10ng/ml
DSPE-PEG(2000)-DBCO is soluble in chloroform and hot water 5mg/ml
DSPE-PEG(2000)-Maleimide is soluble in chloroform 10mg/ml
以上资料源于西安凯新生物科技有限公司
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