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5-羧基四甲基罗丹明琥珀酰亚胺,150810-68-7 生化试剂

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更新时间:2023-07-13 09:17:17浏览次数:469次

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5-羧基四甲基罗丹明琥珀酰亚胺,150810-68-7是在用的多种不同共轭物中Z受欢迎的荧光试剂之一,可标记蛋白质和寡核苷酸。TAMRA具有吸光的性质,它的光谱与几类常用的荧光化合物重叠,TMR是明亮的橙色荧光基团。

5-羧基四甲基罗丹明琥珀酰亚胺,150810-68-7

Although the mixed isomers of 5(6)-TAMRA, SE, also know as 5(6)-TAMRA NHS Ester is a preferred, routinely used red fluorescent dye for staining proteins, labeling peptides and nucleotides, purification of 5(6)-TAMRA labeled peptide and nucleotides might be troublesome due to significant signal broadening in HPLC purification. Peptides and nucleotides labeled with a single isomer TAMRA usually give better resolution in HPLC purification that is often required in the conjugation processes. 5-TAMRA is more often used for labeling peptides and proteins. 6-TAMRA is predominay used for labeling nucleotides and sequencing nucleic acids. 5-TAMRA dye is a bright fluorescent label is compatible with various excitation sources including mercury arc, tungsten and xenon arc lamps, the 544 nm line of the Helium-Neon laser and the 532 nm green laser line.

结构式:

5-羧基四甲基罗丹明琥珀酰亚胺,150810-68-7

Abs/Em Maxima

545/576 nm

Extinction coefficient

78,000

Flow cytometry laser line

532 555 or 568 nm

Microscopy laser line

532 or 555 nm

Spectrally similar dyes

Alexa Fluor® 546, Atto™ 543, CF™ 555 Dye, DyLight™549

Molecular weight

527.53

CAS

150810-68-7

Solubility

DMSO, DMF, MeOH

Purity

>95% (HPLC)

Appearance

Dark red solid

Storage conditions

-20°C. Desiccate

Shipping conditions

Ambient temperature

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