��Ʒ��:΢�ṹ��ˮ��MICROSTRUCTURED HYDROGELS��3Dϸ��װ��壨SMART sphero PLATES��MICROWELLS FOR SPHEROID ASSEMBLY��,��ά��״��֯��壨SMART[contractility] PLATES��ASSEMBLY OF RING-SPHAPED ORGANOIDS��Ʒ��:��4dcell��:MICROSTRUCTURED HYDROGELS�۸�:ѯ����ϵ��:���绰:

4DCELLˮ��

ʹ��΢�ṹ��׵�3Dϸ��

��ƬCOVERSLIPS HYDROGELS

��Ƭ

΢�ṹ��ƬMICROSTRUCTURED HYDROGELS

SMART[sphero] PLATES

΢��װ��MICROWELLS FOR SPHEROID ASSEMBLY

SMART[contractility] PLATES

A3dϸ��ۼ��װ�ɻ�,��״��SSEMBLY OF RING-SPHAPED ORGANOIDS

��

һ��SMART[��]�� ״��ٵ��װ,��鿴��ϸ

ϸ��ڴ��΢�׵Ļ��,΢�״��,�ܹ��Է��γɻ��

>ͨ��ˮ��ı��β��

> ��ڰ�Ĳ��б��ԭλ

> ��һ��,��ӵ��С��ֵ��(zui�� 21 ��)

>��׼��ϸ��ʵ��

> ��ĸնȿ��Ե��

>ʹ�ü�ֱ��,��Ϊϸ��ֱ�ӽ��֮��,��Է��γɻ�״ϸ��ۼ��

> ��κ�ϸ��(�ǲ�Ƭ��󡢶�װ�)

>��Ĺ�ѧ͸��ʹ��Щ��߷ֱ��ʹ�ѧ��΢��ϵͳ��

>��붨��Щ��,��ϵ

��SMART[sphero] �� װ��װ�,��鿴��ϸ

ϸ��ڴ��΢�׵Ļ��,��Է��γ��״��

> ��Ĵ�С��ȡ��ϸ��

> ΢��һ��Ӵ��,ʹ��ڲ��б��ê��

> ��һ��,��ӵ��ߴ��ֵ��(zui�� 91 ��)

>��׼��ϸ��ʵ��

>ʹ�ü�ֱ��,��Ϊϸ��ֱ�ӽ��֮��,��Է��γ��ʽ��ϸ��ۼ��

> ��κ�ϸ��(�ǲ�Ƭ��󡢶�װ�)

>��Ĺ�ѧ͸��ʹ��Щ��߷ֱ��ʹ�ѧ��΢��ϵͳ��

>��΢��ȷʵ��ľ�ȸ��,��ϵ

ˮ��

΢�ṹ��

ϸ�� 3D ��(��ͬ�ն�,��ƽ��΢�ṹ,��ģ���� ��)��

>��ĵĸնȿ��Դӷǳ�� (1 kPa) ��ǳ�Ӳ (50 kPa) ֮��ѡ��

>��ṩ��ֵ��ò(ƽ�ס�Բ�ס��ס��۵�)

>��ĵ��׼��ϸ��ʵ��

>ʹ�ü�ֱ��,��Ϊϸ��ֱ�ӽ��֮��(��Ʒ�Ǩ��ϸ��)

>ԤͿ�� ECM ��(��)

>��κ�ϸ��(�ǲ�Ƭ��󡢶�װ�)

>��Ĺ�ѧ͸��ʹ��Щ��߷ֱ��ʹ�ѧ��΢��ϵͳ��

> ��Ҫ��,��ϵ��

Ӧ�ó��

ˮ��ṹ��

ϸ��ֻ��ϸ��ѧ��ϸ��ԡ�ϸ��Ǩ�ơ��֯��̬��3Dϸ��״��Ƶȡ�

��΢�ṹ��ϵ� HeLa ϸ��

С��̥��άϸ��ڹ��

̽��Ӧ��ʾ��

> 3D ��С�ܲⶨ:3D ��е��γ�

> 3D �ļ�ϸ��ⶨ:�� iPSC-CM ��

SMART[Sphero] �� C ��װ

��γɡ��֯�ṹ��ź�ͨ·��߼��ȡ�

��ھ��΢�׵� PEG ˮ��ʱ,MSC ϸ��γ��״��

��ھ��΢�׵� PEG ˮ��ʱ,MSC (GFP) ϸ��γ��״��

̽��Ӧ��ʾ��

> ��ο��Ƶ��Ĵ�С

>��/��Ⱥ��ϸ��ֳ

��

>�� 3D ϸ��Ļ��ļ��

��[��]�塪��״�л��װ

��ٵ��γɡ��֯�ṹ�ȡ�

С��̥��άϸ��,��ֺ� 22 Сʱ

С��̥��άϸ��,��ֺ� 12 �졣

��

ճ��ӽӺϵ��ϸ��ϵ�Ĺ��ģʽThomas��,2021 ,Developmental Cell 56,1498�C1511
��Ϊ�о��΢��ǰ;��ģ��Polonchuk, L., et al. ��ѧ��, 2017 7 (1), 7005
��ǰ��˵��඾�Լ�ⷽ��
Khouri, MG, et al. δ��ಡѧ, 2015 11 (4), 471-484
Bioinspired living structural color ˮ��Fu, F., et al. ��ѧ��, 2018 3 (16), eaar8580
��пɵ��е��ܵ�ˮ��ʵ��Ʊ�Tse, JR, & Engler, AJ Current protocols in cell biology , 2010 47 (1) 10-16
�� 3D ��ά��ά�غ�͸��-��ˮ��н��ϸ��ϵ�ֻ�Ϊ��Malinen, MM, et al. ��, 2014 35 (19), 5110-5121.