详细介绍

概述
在实际的蛋白分离、分析工作中，广大科研工作者经常会遇到某些疏水性蛋白及聚合物改性蛋白在常规SEC柱上出峰状态与理论不符的情况。针对此种情况，赛分科技在SRT系列色谱柱的基础上，专门推出针对该类样品的Sepax SRT-C SEC系列色谱柱。该键合固定相采用的表面修饰技术，通过在高纯度的硅胶基质上键合均匀的纳米厚度中性亲水涂层而制备得到。由于采用可控的化学修饰技术，确保了填料批间有着可靠的重现性。SEC填料采用化学键合技术，表面亲水涂层覆盖*，因此不仅具有优异的稳定性，而且对蛋白等生物样品的非特异性吸附作用也非常小。同时，大的孔体积保证了高的载样量及优异的分辨率。

技术参数：

固定相	SRT-C SEC-150	SRT-C SEC-300	SRT-C SEC- 500
材料	Neutral, hydrophilic film bonded silica	Neutral, hydrophilic film bonded silica	Neutral, hydrophilic film bonded silica
颗粒大小	5 mm	5 mm	5 mm
孔径 (Å)	~ 150	~ 300	~ 500
蛋白质分子量范围	500 - 150,000	5,000 – 1,250,000	15,000 – 5,000,000
水溶性聚合物分子量范围	500-25,000	1,000-100,000	25,000-500,000
pH 稳定性	2 – 8.5 (pH 8.5-9.5 can be tolerated temporarily.)	2 – 8.5 (pH 8.5-9.5 can be tolerated temporarily.)	2 – 8.5 (pH 8.5-9.5 can be tolerated temporarily.)
反压(psi for a 7.8x300 mm)	~ 700	~ 700	~ 700
压力 (psi)	~ 4,500	~ 3,500	~ 3,000
盐浓度范围	20 mM - 2.0 M	20 mM - 2.0 M	20 mM - 2.0 M
使用温度 (^oC)	~ 80	~ 80	~ 80
流动相兼容性	Aqueous and organic	Aqueous and organic	Aqueous and organic

订购信息

Sepax SRT-C SEC-150

产品名称	粒度	孔径	规格	订货号
SRT-C SEC-150	5um	150 Å	21.5x600mm	235150-21560
SRT-C SEC-150	5um	150 Å	21.5x300mm	235150-21530
SRT-C SEC-150	5um	150 Å	7.5x600mm	235150-7560
SRT-C SEC-150	5um	150 Å	7.8x300mm	235150-7830
SRT-C SEC-150	5um	150 Å	7.8x150mm	235150-7815
SRT-C SEC-150	5um	150 Å	7.8x50 (Guard)	235150-7805
SRT-C SEC-150	5um	150 Å	4.6x300mm	235150-4630
SRT-C SEC-150	5um	150 Å	4.6x150mm	235150-4615
SRT-C SEC-150	5um	150 Å	4.6x50 (Guard)	235150-4605

Sepax SRT-C SEC-300

产品名称	粒度	孔径	规格	订货号
SRT-C SEC-300	5um	300 Å	21.5x600mm	235300-21560
SRT-C SEC-300	5um	300 Å	21.5x300mm	235300-21530
SRT-C SEC-300	5um	300 Å	7.5x600mm	235300-7560
SRT-C SEC-300	5um	300 Å	7.8x300mm	235300-7830
SRT-C SEC-300	5um	300 Å	7.8x150mm	235300-7815
SRT-C SEC-300	5um	300 Å	7.8x50 (Guard)	235300-7805
SRT-C SEC-300	5um	300 Å	4.6x300mm	235300-4630
SRT-C SEC-300	5um	300 Å	4.6x150mm	235300-4615
SRT-C SEC-300	5um	300 Å	4.6x50 (Guard)	235300-4605

Sepax SRT-C SEC-500

产品名称	粒度	孔径	规格	订货号
SRT-C SEC-500	5um	500 Å	21.5x600mm	235500-21560
SRT-C SEC-500	5um	500 Å	21.5x300mm	235500-21530
SRT-C SEC-500	5um	500 Å	7.5x600mm	235500-7560
SRT-C SEC-500	5um	500 Å	7.8x300mm	235500-7830
SRT-C SEC-500	5um	500 Å	7.8x150mm	235500-7815
SRT-C SEC-500	5um	500 Å	7.8x50 (Guard)	235500-7805
SRT-C SEC-500	5um	500 Å	4.6x300mm	235500-4630
SRT-C SEC-500	5um	500 Å	4.6x150mm	235500-4615
SRT-C SEC-500	5um	500 Å	4.6x50 (Guard)	235500-4605